Sentences with phrase «electroporation method»
Compared to conventional microinjection, the electroporation method was easy to carry out, fast and scalable.
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«New innovative method for delivering genes into cells: Modification of cell physiological function with novel parallelized electroporation of mammalian cells by using electrostatic manipulation in a water - in - oil droplet.»
However, most commercial electroporation - based transfection methods require the use of specialized pulse generators to produce short electrical pulses at high voltage.
They then used a method known as electroporation, in which cells are briefly exposed to an electrical field that makes their membranes more permeable, to quickly deliver these RNPs to the interior of the cells.
Whether a protocol uses cell culture methods to expand edited cells for downstream assays, to increase the efficiency of lentiviral transduction or to let cells «rest» after electroporation, optimized culture conditions are key to maintaining cell viability and biological function.
1 - 4 days following DNA injection and electroporation, embryonic brains are dissected, sliced, placed on a filter in culture medium and live imaged over night (Baffet et al., Methods Cell Biol., 2015).
In this study we review the most commonly used indel detection methods and using a robust, sensitive, and cost efficient Indel Detection by Amplicon Analysis method, we have investigated the impact of the most commonly used CRISPR / Cas9 delivery formats, including lentivirus transduction, plasmid lipofection, and ribo nuclear protein electroporation, on the dynamics of indel profile formation.