Sentences with phrase «electroporation of»

Additionally, the authors tested for cellular specificity by using as a convenient experimental framework in utero electroporation of a plasmid that allowed co-expression of the PLE enzyme and of a fluorescent tag (mCherry).

Electroporation of investigator - provided construct with 10 - 15 colonies picked for analysis / Service Request form.

Interim phase II results [12] of first treatment with electroporation of IL - 12 in 28 patients with advanced melanoma, after 24 weeks of treatment, reported at the American Society of Clinical Oncology 2014 Annual Meeting, revealed a 32.2 % objective response rate (ORR; the primary endpoint), with a CR in 10.7 %.

Systemic antitumor effect and clinical response in a phase 2 trial of intratumoral electroporation of plasmid interleukin - 12 in patients with advanced melanoma.

Electroporation of investigator - provided construct into mouse embryonic stem cells / Service Request form plus documentation.

C, Typical number of iPSC colonies obtained with electroporation of pCEP - Oct4 and Nanog or with Oct4 alone.

Electroporation of cultured cells was carried out using the Neon Transfection System (Invitrogen).

Produced by electroporation of MT - 2 cells with pxxHIV - 1Lai proviral DNA.

H. Efficient CRISPR / Cas9 - Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease.

«New innovative method for delivering genes into cells: Modification of cell physiological function with novel parallelized electroporation of mammalian cells by using electrostatic manipulation in a water - in - oil droplet.»

Not exact matches

DeWitt places a plastic tray about the size of a microwave dinner, with 16 thimble - sized wells filled with the culture, under the hood of the electroporation device.

However, most commercial electroporation - based transfection methods require the use of specialized pulse generators to produce short electrical pulses at high voltage.

In addition, the size of the droplet is quite small compared with that in conventional bulk electroporation, resulting in the use of fewer materials in high - throughput analysis.»

Associate Professor Rika Numano explained, «The W / O droplet electroporation technique has several advantages over conventional transfection techniques: small cell number required, as low as 1000 cells; small amount of DNA required, which makes it applicable for various cell types including neural cells; and changeable genome DNA.

They then used a method known as electroporation, in which cells are briefly exposed to an electrical field that makes their membranes more permeable, to quickly deliver these RNPs to the interior of the cells.

The article, «Retrobulbar Inflammation, Adipogenesis and Acute Orbital Congestion in a Preclinical Female Mouse Model of Graves» Orbitopathy Induced by Thyrotropin Receptor Plasmid - in Vivo Electroporation,» will be published in the September issue of Endocrinology.

The amenability of the chick embryo to manipulation and imaging, combined with the powerful technique of in vivo electroporation and the strong similarities of muscle formation in birds and mammals provide a unique paradigm to characterize this process in amniotes.

Expansion of embryonic and adult neural stem cells by in utero electroporation or viral stereotaxic injection.

To generate your own edited clonal hiPS cell lines, try one of our complete systems containing a Cas9 - sgRNA RNP complex delivery system (choose electroporation - based or gesicle - based delivery) and the Cellartis iPSC Single - Cell Cloning DEF - CS Culture Media Kit:

This group cultured human CD34 + cells in StemSpan ™ medium and cytokines as a pre-stimulation step ahead of electroporation, before xenotransplantation into mice or additional in vitro culture.

Whether a protocol uses cell culture methods to expand edited cells for downstream assays, to increase the efficiency of lentiviral transduction or to let cells «rest» after electroporation, optimized culture conditions are key to maintaining cell viability and biological function.

Ex vivo cortical electroporation (E15 mouse embryos) coupled with organotypic culture for 5 days results in radial migration of EGFP + pyramidal neurons to the cortical plate (CP) and the projection of their axon in the intermediate zone (IZ).

Synaptic reconstruction of all excitatory inputs made onto a single cortical pyramidal neuron labeled with sparse in utero electroporation (Unpublished, Dan Iascone)

Synaptic reconstruction of all excitatory and inhibitory inputs made onto a single cortical pyramidal neuron labeled with sparse in utero electroporation.

Though speaking of which, I should go ask if electroporation counts for the «electrical shocking of an organism» badge...

When tested in retinal electroporation studies, DsRed reporter expression was induced in ~ 76 % of GFP + cells, and 100 % of DsRed + cells also expressed GFP.

Important reports from the Weiner lab include the first DNA vaccine studied for HIV as well as for cancer immune therapy of cutaneous T cell lymphoma, the early development of DNA encoded genetic adjuvants, including IL - 12, advances in gene optimization, and advances in electroporation technologies resulting in improved gene delivery.

HV cells overexpressing Nanog were generated by electroporation with a vector containing the Nanog cDNA under the control of a CAG promoter and upstream of IRES Puro cassette followed by selection in puromycin (2 µg / ml) for 2 wk.

The repository of available techniques includes microinjection, particle bombardment («gene gun» technology), laser irradiation, electroporation, sonoporation, and magnetofection (Table 1).

For a typical library, Addgene usually does four to eight electroporation reactions using a total of ~ 400ng of library DNA.

In this study we review the most commonly used indel detection methods and using a robust, sensitive, and cost efficient Indel Detection by Amplicon Analysis method, we have investigated the impact of the most commonly used CRISPR / Cas9 delivery formats, including lentivirus transduction, plasmid lipofection, and ribo nuclear protein electroporation, on the dynamics of indel profile formation.

Faculty research interests and expertise include mechanisms of metastasis in cancer; gene therapy (including electroporation) in cancer; cell and molecular biology of tooth eruption; pharmacology and toxicology of aquatic species; environmental and ecological toxicology; cellular ultrastructure; environmental risk assessment; analytical pharmacology and toxicology; diagnostic neurophysiology; pharmacology of pain and inflammation; exercise physiology; xenobiotic metabolism and disposition; neurochemistry; pulmonary pharmacology; cardiovascular disease; and cetacean morphology.