Sentences with phrase «electroporation with»

HV cells overexpressing Nanog were generated by electroporation with a vector containing the Nanog cDNA under the control of a CAG promoter and upstream of IRES Puro cassette followed by selection in puromycin (2 µg / ml) for 2 wk.

HV cells constitutively expressing the LacZ gene were generated by electroporation with a vector containing a CAG driven β - Geo cDNA followed by selection in G418 (150 µg / ml) for 2 wk.

A cortical layer 2/3 neuron at 21 days in vitro labeled by ex utero electroporation with fluorescent marker for spine visualization.

Not exact matches

DeWitt places a plastic tray about the size of a microwave dinner, with 16 thimble - sized wells filled with the culture, under the hood of the electroporation device.

«New innovative method for delivering genes into cells: Modification of cell physiological function with novel parallelized electroporation of mammalian cells by using electrostatic manipulation in a water - in - oil droplet.»

In addition, the size of the droplet is quite small compared with that in conventional bulk electroporation, resulting in the use of fewer materials in high - throughput analysis.»

The amenability of the chick embryo to manipulation and imaging, combined with the powerful technique of in vivo electroporation and the strong similarities of muscle formation in birds and mammals provide a unique paradigm to characterize this process in amniotes.

Produced by electroporation of MT - 2 cells with pxxHIV - 1Lai proviral DNA.

Ex vivo cortical electroporation (E15 mouse embryos) coupled with organotypic culture for 5 days results in radial migration of EGFP + pyramidal neurons to the cortical plate (CP) and the projection of their axon in the intermediate zone (IZ).

Synaptic reconstruction of all excitatory inputs made onto a single cortical pyramidal neuron labeled with sparse in utero electroporation (Unpublished, Dan Iascone)

Synaptic reconstruction of all excitatory and inhibitory inputs made onto a single cortical pyramidal neuron labeled with sparse in utero electroporation.

C, Typical number of iPSC colonies obtained with electroporation of pCEP - Oct4 and Nanog or with Oct4 alone.

With intratumoral electroporation, tumor cell pores are opened when a mild electrical current is introduced directly through an electrode.

Systemic antitumor effect and clinical response in a phase 2 trial of intratumoral electroporation of plasmid interleukin - 12 in patients with advanced melanoma.

Interim phase II results [12] of first treatment with electroporation of IL - 12 in 28 patients with advanced melanoma, after 24 weeks of treatment, reported at the American Society of Clinical Oncology 2014 Annual Meeting, revealed a 32.2 % objective response rate (ORR; the primary endpoint), with a CR in 10.7 %.

Electroporation of investigator - provided construct with 10 - 15 colonies picked for analysis / Service Request form.