Sentences with word «elution»

Usually, 1 ml of elution buffer requires 50 — 100 μl of saturated phosphate buffer depending on the temperature.

Complexes were eluted by adding 250 µl of elution buffer [1 % SDS, 0.1 M NaHCO3] to pelleted beads and vortexed for 30 minutes.

A first - in - human study of a new polymer - free drug - filled stent, which provides controlled drug elution from an internal lumen, indicated non-inferior in - stent late lumen loss at nine - months compared with historical zotarolimus - eluting stent (Resolute) data.

The polymer - free drug - filled stent (DFS) used in the study was designed to provide controlled and sustained drug elution from an internal stent lumen without a polymer coating.

Elute the antibodies with 6 ml elution buffer and neutralize the solution with saturated phosphate buffer.

Frequently used for western blots, IP, co-IP, IF, flow - cytometry, but rarely used for purification as elution requires low pH

In vitro elution of amikacin and vancomycin from impregnated plaster of Paris beads.

Small laser - drilled holes on the abluminal stent surface control drug elution.

The supernatant was mixed with 300 µl of Ni - NTA agarose (Qiagen), and the proteins were allowed to bind for 3 hours, followed by washing with 20 ml of resuspension buffer, and eluted with 500 µl of elution buffer (20 mM NaHPO4, 300 mM NaCl, 10 % glycerol, 150 mM Imidazole, pH 7.8).

The plate was washed with 1.6 mL of binding buffer and proteins eluted with 0.2 mL of elution buffer (20 mM sodium phosphate, 0.4 M imidazole, 0.5 M NaCl, pH 7.4).

A polymer - free metal surface stent that is capable of controlled antiproliferative drug elution may avoid the adverse effects of polymer - induced inflammation, thrombosis and non-uniformity, and could potentially allow for shorter durations of dual antiplatelet therapy.

Elution was repeated and combined.

Samples were first homogenized with a Geno / Grinder and then treated with RNase - free DNase I prior to elution.

The elution positions of CR / VLDL, IDL, LDL, and HDL are indicated.

The elution positions of CR / VLDL, intermediate - density lipoprotein (IDL), LDL, and HDL are indicated.

Approximately 25 mg of each tissue sample was homogenized, DNA was extracted using Qiagen DNeasy kits, the DNA sample was eluted from the column with 200 µl elution buffer, and then the purified DNA / buffer was heated for 5 minutes at 95 degrees.