Sentences with phrase «embryo cultures with»

Embryo growth after 12 months of dry storage was negligible and was observed only in embryo cultures with growth hormones (c. 12 %) and no embryo growth was evident after 3 months of storage at − 18 °C in either medium.

Most notably, however, parthenogenic blastocysts were only produced from embryos cultured with supplementation and not with Sage or IVM - medium alone (Table 2 and Figure S2).

Not exact matches

Visually, she is filming and analyzing time - lapse images of human embryos in the incubator and has been able to correlate various parameters of how cells divide with the probability that the embryos will make it to a full blastocyst stage by day 5 - 6 of culture.

Using her new culture system, she joined forces with colleagues to research which cells in an embryo contribute to which parts of the adult animal, a process called fate - mapping.

Furthermore, genes that are lethal when knocked out in embryos can be analyzed with RNAi in cell culture.

Although a clause in the law that funds NIH prevents the agency from funding research that would harm or destroy an embryo, a lawyer at the Department of Health and Human Services ruled in 1999 that because stem cells — which can grow ad infinitum in culture — are not themselves embryos, the NIH could fund work with cells that were derived by privately funded researchers or researchers overseas.

The growth potential of excised embryos cultured on 1/2 MS medium declined by 31 % after 1 month of dry storage or after 24 h of dry storage at − 18 °C (Table 3), but growth was similar to that of embryos from freshly collected seeds (c. 80 %) when embryos were cultured with growth hormones.

For Noggin protein - treated caps, stage 9 animal cap explants were cultured with 500 nM mouse Noggin protein (Sigma - Aldrich, catalogue number N6784) in 0.7 × MMR and 50 µg / ml gentamicin sulphate and grown to sibling embryo stage 15 for transplantation.

Early development is also studied with respect to in vitro culture of human embryos for IVF and its possible epigenetic effects in the foetus and child.

I disagree with a moratorium, which is in any case unlikely to work well, indeed I am fully supportive of research being carried out on early human embryos in vitro [in culture / in the lab], especially on embryos that are not required for reproduction and would otherwise be discarded.

We exploit chick and mouse embryo animal models, combined with live imaging, cell and tissue cultures and molecular approaches.

Ex vivo cortical electroporation (E15 mouse embryos) coupled with organotypic culture for 5 days results in radial migration of EGFP + pyramidal neurons to the cortical plate (CP) and the projection of their axon in the intermediate zone (IZ).

While the genome editing capabilities without cultured primordial germ - cells is limited and a slower process, the optimization of methods for handling embryos and caring for engineered birds will be instrumental to an efficient de-extinction program as well as genetic rescue of other birds with similar parenting behaviors to pigeons.

Hence, its members favor primary cell lines and try to avoid experiments with cultured cell lines, they favor three - dimensional cell cultures over two - dimensional cell monolayers that are cultivated on hard and flat surfaces and they try to maintain the three - dimensional context of plants, cell clusters, tissues sections and small animal embryos.

Fused reconstructed embryos were further activated in 5 µM ionomycin for 5 min, followed by exposure to 1.9 mM 6 - dimethylaminopurine (DMAP) in synthetic oviduct fluid with amino acids (SOFaa) for 4 h. Following activation, embryos were then transferred and cultured in SOFaa.

One - cell embryos obtained from superovulated female mice mated with (C57BL / 6xCBA) males were cultured in KSOM media (Sigma - Aldrich) supplemented with 10 % FCS (Sigma - Aldrich) for 4 days.

Toward defined physiological embryo culture media: Replacement of BSA with recombinant albumin.

Gardner DK, Rodriegez - Martinez H, Lane M. Fetal development after transfer is increased by replacing protein with the glycosaminoglycan hyaluronan for mouse embryo culture and transfer.

The exact reason for this is unknown, says co-author Michael Chapman, but it likely has to do with the length of time an embryo is cultured in the laboratory.

This technology requires technical skills that are typically not offered by veterinary practices and includes aspiration of immature or mature eggs from mares using ultrasound - guided transvaginal aspiration (TVA) of follicles, in vitro culture of the eggs, micromanipulation and microinjection of eggs with a single selected sperm, and embryo culture in the laboratory, with freezing, and transfer of embryos to synchronized recipient mares.