Sentences with phrase «embryonic cell genomes»

In SIF - seq, hundreds or thousands of DNA fragments to be tested for enhancer activity are coupled to a reporter gene and targeted into a single, reproducible site in embryonic cell genomes.

Not exact matches

The ability of SIF - seq to use reporter assays in mouse embryonic stem cells to identify human embryonic stem cell enhancers that are not present in the mouse genome opens the door to intriguing research possibilities as Dickel explains.

Using a mathematical model known as the Ising model, invented to describe phase transitions in statistical physics, such as how a substance changes from liquid to gas, the Johns Hopkins researchers calculated the probability distribution of methylation along the genome in several different human cell types, including normal and cancerous colon, lung and liver cells, as well as brain, skin, blood and embryonic stem cells.

Derived mostly from human embryonic kidney 293T (HEK293T) and HeLa cell lines, EdiGene Knockout (KO) Cell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target gcell lines, EdiGene Knockout (KO) Cell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target gCell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target gene.

Mitchell and her colleagues eliminated this possibility when they deleted these nearby regions in the genome of mice and found there was no impact on the gene's ability to be turned on in embryonic stem cells.

«We studied how the Sox2 gene is turned on in mice, and found the region of the genome that is needed to turn the gene on in embryonic stem cells,» said Professor Jennifer Mitchell of U of T's Department of Cell and Systems Biology, lead invesigator of a study published in the December 15 issue of Genes & Development.

«Though the degree to which human embryonic stem cells possess this feature is not entirely clear, by understanding how another complex organism's genome works we ultimately learn more about how our own genome works,» said Zhou.

Next, they found that the genomes of naïve embryonic stem cells have less methylation — the addition of methyl chemical groups along DNA.

Not only did a high percentage of embryonic cells get repaired, but also gene correction didn't induce any detectable off - target mutations and genome instability — major concerns for gene editing.

An ambitious plan to map the genome's regulatory elements will focus first on mouse embryonic stem cells.

The study, published today in the journal Cell Stem Cell and led by Dr. Shunsuke Ishii from RIKEN, sought to identify the molecule in the mammalian oocyte that induces the complete reprograming of the genome leading to the generation of totipotent embryonic stem cells.

They accomplished this by using mouse embryonic stem cells to manipulate the mouse genome at the very start of development.

Intact genome from one particular mouse embryonic stem cell.

The researchers have illustrated the structure in accompanying videos, which show the intact genome from one particular mouse embryonic stem cell.

Research led by the Babraham Institute with collaborators in the UK, Canada and Japan has revealed a new understanding of how an open genome structure supports the long - term and unrestricted developmental potential in embryonic stem cells.

The IMPC envisages a ten year programme to undertake a broad - based, systematic genome - wide phenotyping project of knockout mice generated from the embryonic stem cell mutant resources developed by the International Knock - out Mouse Consortium (IKMC).

In work published on 24 November in the journal Nature biotechnology, Anselme Perrier's team at I - Stem identified a recurrent anomaly in the genome of human embryonic stem cells left in culture for too long.

On Aug. 3, the scientific article in Nature finally gave us some facts about the much - hyped experiments that involved editing the genomes of human embryos at the Center for Embryonic Cell and Gene Therapy at Oregon Health and Science University.

In partnership with several international programs, the initial five - year phase of KOMP will reach its goal of creating knockout mouse embryonic stem cell lines for each of the approximately 21,000 protein - coding genes in the mouse genome this year.

The Repository of mouse ES cell lines, strains and vectors generated by the trans - NIH KOMP initiative that aims to generate a public resource comprised of mouse embryonic stem (ES) cells containing a null mutation in every gene in the mouse genome.

To conduct the experiments, the team will use state of the art genome engineering technology to generate mutant embryonic stem cells at a large scale, followed by transcriptional profiling via next generation sequencing.

To find those genes, Yamanaka had compared all the genes (the genome) of an embryonic stem cell with those of an adult cell to find genes that were turned on in the ES cell but turned off in the adult cell.

Henk Stunnenberg, leader of one of the research groups that carried out the study and coordinator of both Heroic and the recently started High Impact Project BLUEPRINT, said: «The epigenetic make - up - a layer of regulatory instructions on top of the genome - of the pure embryonic stem cells shows remarkable and unexpected features, in particular with respect to developmental genes.

However, during prolonged culture one of eight embryonic stem cell lines showed a resurgence of the spindle donor's mitochondrial genome.

Stem cell researchers from UCLA used a high resolution technique to examine the genome, or total DNA content, of a pair of human embryonic stem cell lines and found that while both lines could form neurons, the lines had differences in the numbers of certain genes that could control such things as individual traits and disease susceptibility.

A genome - scale RNAi screen for Oct4 modulators defines a role of the Paf1 complex for embryonic stem cell identity.

With the new SIF - seq technique, mouse embryonic stem cells can be used to identify human embryonic stem cell enhancers even when the human enhancers are not present in the mouse genome.

Title: A genome - wide RNAi screen reveals MAP kinase phosphatases as key ERK pathway regulators during embryonic stem cell differentiation (IN PRESS, Oct 2012) Authors: Sharrocks A, Yang S - H, Kalkan T, Haslam C, Smith A Date: IN PRESS, Oct 2012 Publication Details: PLoS Genetics

Reference: September 13, 2007 issue of Cell Stem Cell under the title «Whole - Genome Mapping of Histone H3Lys4 and 27 Trimethylations Reveals Distinct Genomic Compartments in Human Embryonic Stem Cells».

Embryonic stem cells with identical genomes grow into distinctive tissues, such as heart, bone, and brain.

In their latest study, the group conducted a genome wide screening by developing a novel primary mouse embryonic stem cell line that enhances the efficiency of CRISPR - Cas9 mediated mutations.

Topics covered include embryonic stem cells, pluripotency, germline stem cells, tissue - specific stem cells, stem cell differentiation, epigenetics, stem cell genomics and systems biology, genome reprogramming, cancer stem cells, stem cell niches, stem - cell - based disease models, nuclear transfer technology, bioengineering, drug discovery, in vivo imaging of stem cells, therapeutic applications, regenerative medicine, clinical and translational insights, stem cell research policies, ethical issues, and technical or resource - based innovations.

During embryonic development, cells use the information contained in their genome to build the organism.

The scientists had strategically inserted these foreign DNA «markers» at particular points along the genome, next to genes expressed only in embryonic stem cells.

To develop the methodology, the group analyzed the molecular profiles of human embryonic stem cells and compared them with data for 12,000 samples of 33 different tumor types held by The Cancer Genome Atlas (TCGA), a U.S. public database.

The Knockout Mouse Project (KOMP & KOMP2) is a trans - NIH initiative that aims to generate a comprehensive and public resource comprised of mouse embryonic stem (ES) cells containing a null mutation in every gene in the mouse genome.

Here we used genome - saturated mutagenesis to create a biobank of over 100,000 individual haploid mouse embryonic stem (mES) cell lines targeting 16,970 genes with genetically barcoded, conditional and reversible mutations.

In the November 6 Nature Methods, the team led by Bing Lim and Massimo Nichane of the Genome Institute of Singapore and Kyle M. Loh of Stanford University School of Medicine, and including Siva V, Ph.D., of The Jackson Laboratory's Single Cell Biology Lab, showed that mouse Sox9 + multipotent embryonic lung progenitors can be isolated and expanded long - term in 3D culture.