Sentences with phrase «embryonic fibroblasts at»

Latex bead labelling and comparative immunofluorescence analysis of the p62 subunit of the TFIIH was performed as described previously [16,17] using primary mouse embryonic fibroblasts at passages 2 — 5.

Not exact matches

Vermulst's study looked only at tissues from the heart, brain, and embryonic fibroblasts.

In 1963 Pietro Angeletti and one of us (Levi Montalcini), who were then at the Istituto Superiore di Sanità in Rome, dissected sensory and sympathetic ganglia into their cellular components: neurons, glial cells (which support and nourish the neurons) and fibroblasts (embryonic connective - tissue cells).

FINDINGS The researchers identified 32 known imprinted genes, but no new ones, implying that the list of imprinted genes in the mouse — at least in embryonic fibroblasts — is nearly complete, says Morison, who was not involved in the study (Nucleic Acids Res, 42:1772 - 83, 2014).

For instance, MEF cells are usually made of fibroblasts from the mouse embryos at embryonic day 13.5 and only cells at early passages (p2 to p3) are used as feeders for derivation and culture of embryonic stem (ES) and iPS cells.

The skin biopsies were washed in Ca / Mg - free Dulbecco's Phosphate Buffered Saline (PBS, Invitrogen, Carlsbad, CA, http://www.invitrogen.com) and minced into approximately 12 smaller pieces before being seeded onto gelatin - coated 6 - well cell culture plates (Corning Enterprises, Corning, NY, http://www.corning.com) containing mouse embryonic fibroblast (MEF) media consisting of Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10 % fetal bovine serum (FBS, Invitrogen) and 100 IU / ml penicillin - streptomycin (Invitrogen), and cultured at 37 °C in a 5 % CO2 incubator.