Shi, Y.; Desponts, C.; Do, J. T.; Hahm, H. S.; Scholer, H. R.; Ding, S. Induction of pluripotent stem cells from mouse
embryonic fibroblasts by Oct4 and Klf4 with small - molecule compounds.
Not exact matches
Cyclooxygenase - 2 (COX - 2) was highly elevated
by capsaicin treatment in tumors and murine
embryonic fibroblasts from TRPV1 / KO mice.
Imagine researchers» excitement, then, when Kyoto University's Shinya Yamanaka reported last year that his group had created embryolike cells directly, without transferring chromosomes, simply
by injecting mouse
fibroblast cells with four genes that are active in
embryonic cells but not in adult ones.
Human
embryonic stem cells derived from affected embryos during a pre-implantation diagnostic (PGD), as well as the conversion of somatic cells, such as skin
fibroblasts, into induced pluripotent stem cells
by genetic manipulation, offer the unique opportunity to have access to a large spectrum of disease - specific cell models.
It has recently been demonstrated that mouse and human
fibroblasts can be reprogrammed into an
embryonic stem cell - like state
by introducing combinations of four transcription factors.
«Induction of Pluripotent Stem Cells From Mouse
Embryonic and Adult
Fibroblast Cultures
by Defined Factors,»
by Kazutoshi Takahashi and Shinya Yamanaka, Cell, Aug. 25, 2006.
They made adult fibroblastic mouse cells become essentially mouse
embryonic stem cells, in appearance and function,
by forcing the
fibroblasts to express four key
embryonic stem cell factors: Oct - 4, Sox2, Klf4, and c - Myc.
(6) Moreover, subcutaneous adipose of prematurely - aged transgenic mice exhibited high levels of staining for the senescence marker senescence - associated - β - galactosidase (SAβ - gal) and expressed high levels of several established markers of senescence, including p21, p19, interleukin - 6, (insulin - like growth factor binding protein - 2 (Igfbp2), and Pai - 1; primary BubR1H / H; INK - ATTAC mouse
embryonic fibroblasts forced artificially into senescence
by oncogenic Ras or serial passage exhibited a subpopulation that was both GFP + and stained positively SAβ - gal.
HuH - 7 (human HCC), HepG2 (human hepatocellular blastoma), 293T (human
embryonic kidney
fibroblast), HeLa (human cervical cancer cells), NIH 3T3 (murine
fibroblast), and HCT116 (human colon cancer) and its derived cell line HCT116 p21 − / − (kindly provided
by Dr. B. Vogelstein, Howard Hughes Medical Institute, Chevy Chase and the Sydney Kimmel Comprehensive Cancer Center of the Johns Hopkins University School of Medicine, Baltimore, MD; ref.
The latest study builds on earlier research from Izpisúa Belmonte's laboratory showing that extra limbs could be induced to grow in
embryonic chicks
by implanting beads soaked in
fibroblast growth factor (FGF).
By observing the cells in this system, they found that the embryonic cardiac fibroblasts promoted cell division by the cardiomyocytes more efficiently than adult cardiac fibroblast
By observing the cells in this system, they found that the
embryonic cardiac
fibroblasts promoted cell division
by the cardiomyocytes more efficiently than adult cardiac fibroblast
by the cardiomyocytes more efficiently than adult cardiac
fibroblasts.
The novel reprogramming approach employed the retroviral transduction of Oct4, Sox2, and Klf4 in mouse
embryonic and adult
fibroblasts followed
by treatment with the GSK3β Inhibitor BIO and the pleiotropic cytokine Oncostatin M (OSM).