Sentences with phrase «embryonic fibroblasts on»

Not exact matches

Hayashi then drew on other research showing that one key regulatory molecule (activin A) and a growth factor (basic fibroblast growth factor) could convert cultured early embryonic stem cells into cells akin to epiblasts.

The latest study builds on earlier research from Izpisúa Belmonte's laboratory showing that extra limbs could be induced to grow in embryonic chicks by implanting beads soaked in fibroblast growth factor (FGF).

Embryonic cardiac fibroblasts promote myocyte proliferation, while adult fibroblasts promote myocyte hypertrophy,» said Masaki Ieda, MD, PhD, Gladstone postdoctoral fellow and lead author on the study.

Most prior established iPS cell lines were derived and maintained on mouse embryonic fibroblast (MEF) cells supplemented with exogenous leukemia inhibitory factor (LIF).

Cells were cultured on irradiated mouse embryonic fibroblasts (MEF) in DMEM / F12 culture medium supplemented with KnockOut serum replacer (20 %), non-essential amino acids (0.1 mM), L - glutamine (1 mM), ß - mercaptoethanol (0.1 mM) and 100 ng / ml zebrafish basic fibroblast growth factor.

Briefly, ES colonies were maintained on mitotically inactivated mouse embryonic fibroblasts (density of 60,000 cells / cm2) in media consisting of DMEM (Invitrogen cat.

hESC lines I6 and H9 were maintained on Matrigel (BD Biosciences, Bedford, MA; http://www.bdbiosciences.com) coated dishes in medium (comprised of Dulbecco's Modified Eagle's Medium / Ham's F12 supplemented with 20 % knockout serum replacement (KSR), 2 mM non-essential amino acids, 4 mM L - glutamine, 0.1 mM β - mercaptoethanol, 50 µg / ml Penn - Strep, and 4 ng / ml of basic fibroblast growth factor) conditioned with mouse embryonic fibroblasts for 24 hours as previously described [9], [10].

But initial experiments on human fibroblasts from three sources — fetal heart cells, embryonic stem cells and neonatal skin cells — revealed that the GMT combination alone was not sufficient.