Sentences with phrase «embryonic mouse fibroblasts»

The specific fibroblasts used were embryonic mouse fibroblasts.

The conversion rate was lower, but the induced neurons seemed just as much like their natural counterparts as those produced from embryonic mouse fibroblasts.

They found that the combination of the transcription factors Brn3a plus Ngn1, or Brn3a plus Ngn2, reprogrammed a significant percentage of the embryonic mouse fibroblasts into what looked — and acted — like mature DRG - type sensory neurons.

Not exact matches

Cyclooxygenase - 2 (COX - 2) was highly elevated by capsaicin treatment in tumors and murine embryonic fibroblasts from TRPV1 / KO mice.

Repeated transfection of two expression plasmids, one containing the complementary DNAs (cDNAs) of Oct3 / 4, Sox2, and Klf4 and the other containing the c - Myc cDNA, into mouse embryonic fibroblasts resulted in iPS cells without evidence of plasmid integration, which produced teratomas when transplanted into mice and contributed to adult chimeras.

For this study, Guttridge, first author David J. Wang, who developed many of the study's concepts, and their colleagues monitored NF - kB activity during tumor development using mouse embryonic fibroblasts and two mouse models.

Imagine researchers» excitement, then, when Kyoto University's Shinya Yamanaka reported last year that his group had created embryolike cells directly, without transferring chromosomes, simply by injecting mouse fibroblast cells with four genes that are active in embryonic cells but not in adult ones.

In vitro experiments used human colon carcinoma cells with and without MMP9 and mouse embryonic fibroblasts, which are connective tissue cells that make the extracellular matrix and collagen and play an important role in tissue repair.

Expanding from their previous studies with mice, the researchers first established that under specific conditions, culturing human embryonic stem cells with fibroblast growth factor 2 (FGF2) leads to neural differentiation particular to the midbrain / hindbrain region — the location of the cerebellum — within three weeks, and the expression of markers for the cerebellar plate neuroepithelium — the part of the developing nervous system specific for the cerebellum — within five.

Latex bead labelling and comparative immunofluorescence analysis of the p62 subunit of the TFIIH was performed as described previously [16,17] using primary mouse embryonic fibroblasts at passages 2 — 5.

Chimeric mice and mouse embryonic fibroblasts were generated according to standard procedures.

It has recently been demonstrated that mouse and human fibroblasts can be reprogrammed into an embryonic stem cell - like state by introducing combinations of four transcription factors.

«Induction of Pluripotent Stem Cells From Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors,» by Kazutoshi Takahashi and Shinya Yamanaka, Cell, Aug. 25, 2006.

They made adult fibroblastic mouse cells become essentially mouse embryonic stem cells, in appearance and function, by forcing the fibroblasts to express four key embryonic stem cell factors: Oct - 4, Sox2, Klf4, and c - Myc.

(6) Moreover, subcutaneous adipose of prematurely - aged transgenic mice exhibited high levels of staining for the senescence marker senescence - associated - β - galactosidase (SAβ - gal) and expressed high levels of several established markers of senescence, including p21, p19, interleukin - 6, (insulin - like growth factor binding protein - 2 (Igfbp2), and Pai - 1; primary BubR1H / H; INK - ATTAC mouse embryonic fibroblasts forced artificially into senescence by oncogenic Ras or serial passage exhibited a subpopulation that was both GFP + and stained positively SAβ - gal.

Valdivia submitted this image of mouse embryonic fibroblasts forming focal adhesions, points of contact of the cell with the extracellular matrix.

FINDINGS The researchers identified 32 known imprinted genes, but no new ones, implying that the list of imprinted genes in the mouse — at least in embryonic fibroblasts — is nearly complete, says Morison, who was not involved in the study (Nucleic Acids Res, 42:1772 - 83, 2014).

TEST CASE RESEARCHER Piroska Szabó, Associate Professor, Center for Epigenetics, Van Andel Research Institute ORGANISM Mouse METHOD RNA - seq In 2014, Szabó and colleagues reported how they'd used mouse embryonic fibroblasts to test whether the relatively new method of RNA sequencing could reveal known and novel imprinted gMouse METHOD RNA - seq In 2014, Szabó and colleagues reported how they'd used mouse embryonic fibroblasts to test whether the relatively new method of RNA sequencing could reveal known and novel imprinted gmouse embryonic fibroblasts to test whether the relatively new method of RNA sequencing could reveal known and novel imprinted genes.

Cell Stem Cell «Recently three different studies were published demonstrating that mouse fibroblast (skin) cells can be directly reprogrammed to behave like embryonic stem cells.»

For instance, MEF cells are usually made of fibroblasts from the mouse embryos at embryonic day 13.5 and only cells at early passages (p2 to p3) are used as feeders for derivation and culture of embryonic stem (ES) and iPS cells.

With current methodologies, mouse embryonic fibroblast (MEF) cells have been used as a feeder layer to derive both mouse and human iPS cells.

The novel reprogramming approach employed the retroviral transduction of Oct4, Sox2, and Klf4 in mouse embryonic and adult fibroblasts followed by treatment with the GSK3β Inhibitor BIO and the pleiotropic cytokine Oncostatin M (OSM).

Most prior established iPS cell lines were derived and maintained on mouse embryonic fibroblast (MEF) cells supplemented with exogenous leukemia inhibitory factor (LIF).

Shi, Y.; Desponts, C.; Do, J. T.; Hahm, H. S.; Scholer, H. R.; Ding, S. Induction of pluripotent stem cells from mouse embryonic fibroblasts by Oct4 and Klf4 with small - molecule compounds.

(A) The expression patterns of germ cell marker genes in monkey testis (5 years old), mouse embryonic fibroblast (MEF), monkey ES cells (ES), and developing EBs (days 3, 7, 14, 21, and 28) were examined using an RT - PCR analysis.

Cells were cultured on irradiated mouse embryonic fibroblasts (MEF) in DMEM / F12 culture medium supplemented with KnockOut serum replacer (20 %), non-essential amino acids (0.1 mM), L - glutamine (1 mM), ß - mercaptoethanol (0.1 mM) and 100 ng / ml zebrafish basic fibroblast growth factor.

The skin biopsies were washed in Ca / Mg - free Dulbecco's Phosphate Buffered Saline (PBS, Invitrogen, Carlsbad, CA, http://www.invitrogen.com) and minced into approximately 12 smaller pieces before being seeded onto gelatin - coated 6 - well cell culture plates (Corning Enterprises, Corning, NY, http://www.corning.com) containing mouse embryonic fibroblast (MEF) media consisting of Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10 % fetal bovine serum (FBS, Invitrogen) and 100 IU / ml penicillin - streptomycin (Invitrogen), and cultured at 37 °C in a 5 % CO2 incubator.

Briefly, ES colonies were maintained on mitotically inactivated mouse embryonic fibroblasts (density of 60,000 cells / cm2) in media consisting of DMEM (Invitrogen cat.

We transduced our SSEA3 - positive and SSEA3 - negative populations with the same retroviral vectors, under identical experimental conditions, and seeded the transduced cells onto inactivated mouse embryonic fibroblasts (MEFs).

hESC lines I6 and H9 were maintained on Matrigel (BD Biosciences, Bedford, MA; http://www.bdbiosciences.com) coated dishes in medium (comprised of Dulbecco's Modified Eagle's Medium / Ham's F12 supplemented with 20 % knockout serum replacement (KSR), 2 mM non-essential amino acids, 4 mM L - glutamine, 0.1 mM β - mercaptoethanol, 50 µg / ml Penn - Strep, and 4 ng / ml of basic fibroblast growth factor) conditioned with mouse embryonic fibroblasts for 24 hours as previously described [9], [10].

Cells were stained in solution using a mixture of GCTM - 2 (mouse IgM), TG30 (anti-CD9, mouse IgG2a) and Thy1.2 - PE (to gate out any mouse embryonic fibroblasts, BD Bioscience, cat.