Sentences with phrase «embryos cultured for»

She thinks that data from embryos cultured for nearly two weeks in the laboratory will provide more useful information than Egli's stem cell studies.

Human chorionic gonadotropin was detected in the medium surrounding two embryos cultured for more than 7 days after fertilization.

Not exact matches

He was also instrumental in developing techniques for post thaw extended culture (PTEC) following fertilized oocyte cryopreservation demonstrating the importance of embryo - endometrial synchrony for implantation, for which he received another research award.

He is a strong advocate for elective single embryo transfer (eSET) in IVF, and therefore for high standards for culturing and vitrifying embryos.

During the first year, if you should need services such as assisted hatching, embryo freezing and storage, or extended cultures, it is all included — you will not be billed extra for those services.

Normally, clones are grown in culture used for embryos, and few clones flourish.

Researchers have videotaped cultures in which embryos develop but found no visual pattern that hints at which cells are about to sprout, and staining for certain patterns of gene expression has been inconclusive.

When glucose is added to the culture medium for cows and sheep embryos, a greater number of males survive.

The ability to keep human embryos developing in the lab for almost 2 weeks — achieved for the first time this year — should provide new insights into very early human development, and generate debate on whether ethical limits on studying embryos in culture should be extended.

Embryos were extracted and cultured from freshly collected seeds, seeds that had been stored dry at 15 °C and 15 % RH for 1, 3 or 12 months and seeds that had been stored at − 18 °C for 24 h or 3 months after drying to stable moisture content (5 %, determined gravimetrically) for 2 weeks at 15 °C and 15 % RH.

Embryo cultures were maintained at 22 — 24 °C for 8 weeks, in darkness for the first 2 weeks followed by a 16 - h / 8 - h light — dark cycle.

For Noggin protein - treated caps, stage 9 animal cap explants were cultured with 500 nM mouse Noggin protein (Sigma - Aldrich, catalogue number N6784) in 0.7 × MMR and 50 µg / ml gentamicin sulphate and grown to sibling embryo stage 15 for transplantatiFor Noggin protein - treated caps, stage 9 animal cap explants were cultured with 500 nM mouse Noggin protein (Sigma - Aldrich, catalogue number N6784) in 0.7 × MMR and 50 µg / ml gentamicin sulphate and grown to sibling embryo stage 15 for transplantatifor transplantation.

If Obama really wanted to resolve one front of the culture wars and show respect for pro-lifers, as he claimed, he would have refused to make citizens complicit in embryo killing by simply continuing the Bush policies.

They then inserted the spatially oriented human stem cells (human rsPSCs) into specific regions of partially dissected mouse embryos and cultured them in a dish for 36 hours.

Early development is also studied with respect to in vitro culture of human embryos for IVF and its possible epigenetic effects in the foetus and child.

Researchers have discovered a gene in zebrafish so powerful it can be used to redirect the fate of cells in the developing embryo to become beating heart cells, suggesting that a similar gene in humans could be used to generate heart cells in culture for transplant in ailing people.

I disagree with a moratorium, which is in any case unlikely to work well, indeed I am fully supportive of research being carried out on early human embryos in vitro [in culture / in the lab], especially on embryos that are not required for reproduction and would otherwise be discarded.

Embryos were cultured to stage 51, and tested for phototropic behavior.

As a result of these findings, clinicians and embryologists can more quickly identify the healthiest embryo for implantation and reduce the amount of time an embryo is cultured in the laboratory prior to transfer.

Also this technique is only likely to be useful in a small proportion of cycles in which embryos are cultured to the blastocyst stage and multiple embryos are then available for selection.

Finally the fact that these embryos have to be cultured for longer in the laboratory to use this test might in itself be causing the stress which the test then detects, which could in the end be counterproductive.

Our team would like to be able to research passenger pigeon genes for de-extinction while primordial germ cell cultures are developed, and an Australian team has developed an effective way of bypassing cell cultures for engineering birds by going directly to the primordial germ cells in the embryo.

We prefer to culture our cells on mouse embryo fibroblasts (MEFs) but occasionally grow the cells feeder free for particular applications, such as nucleofection, viral transduction or karyotyping.

Ex vivo cortical electroporation (E15 mouse embryos) coupled with organotypic culture for 5 days results in radial migration of EGFP + pyramidal neurons to the cortical plate (CP) and the projection of their axon in the intermediate zone (IZ).

For instance, MEF cells are usually made of fibroblasts from the mouse embryos at embryonic day 13.5 and only cells at early passages (p2 to p3) are used as feeders for derivation and culture of embryonic stem (ES) and iPS celFor instance, MEF cells are usually made of fibroblasts from the mouse embryos at embryonic day 13.5 and only cells at early passages (p2 to p3) are used as feeders for derivation and culture of embryonic stem (ES) and iPS celfor derivation and culture of embryonic stem (ES) and iPS cells.

While the genome editing capabilities without cultured primordial germ - cells is limited and a slower process, the optimization of methods for handling embryos and caring for engineered birds will be instrumental to an efficient de-extinction program as well as genetic rescue of other birds with similar parenting behaviors to pigeons.

Fused reconstructed embryos were further activated in 5 µM ionomycin for 5 min, followed by exposure to 1.9 mM 6 - dimethylaminopurine (DMAP) in synthetic oviduct fluid with amino acids (SOFaa) for 4 h. Following activation, embryos were then transferred and cultured in SOFaa.

Approximately 20 % of oocytes routinely retrieved following hormone stimulation are classified as immature (termed germinal vesicle (GV) or metaphase I (MI) stage); these oocytes are discarded due to their reduced potential for embryo development under current culture conditions [1].

It has been demonstrated that the composition of the medium used for embryo culture has a profound effect on the methylation pattern in the resultant two - cell embryos [10], indicating that, in addition to imprinted genes, other epigenetic alterations may intensely modify gene expression.

The GEM Facility will thaw one straw of frozen embryos and culture overnight to the blastocyst stage to insure that embryo freezing is feasible for that particular mouse line.

One - cell embryos obtained from superovulated female mice mated with (C57BL / 6xCBA) males were cultured in KSOM media (Sigma - Aldrich) supplemented with 10 % FCS (Sigma - Aldrich) for 4 days.

Gardner DK, Rodriegez - Martinez H, Lane M. Fetal development after transfer is increased by replacing protein with the glycosaminoglycan hyaluronan for mouse embryo culture and transfer.

The exact reason for this is unknown, says co-author Michael Chapman, but it likely has to do with the length of time an embryo is cultured in the laboratory.