A team of developmental biologists led by Hans Schöler and Karen Hübner at the University of Pennsylvania placed densely packed clusters of stem cells from mouse
embryos in a petri dish, using fetal calf serum as a growth medium and adding a gene protein that turns green when germ cells form.
Not exact matches
Their technique, which involves taking a woman's mature oocyte (egg) and fertilizing it with sperm
in a
petri dish to form an
embryo, led to a U.K. woman's giving birth to the first «test tube baby»
in 1978.
IVF is the process by which an egg is fertilized by sperm
in a
Petri dish that, if successful, results
in (an)
embryo (s) 3 - 5 days later.
After a few hours dividing
in a
Petri dish, researchers implanted the cloned
embryo into a surrogate mother.
A quarter century ago, British physicians Robert Edwards and Patrick Steptoe faced condemnation when they added an egg to sperm
in a
petri dish and created an
embryo that became Louise Brown.
«We've now devised a technology where we can effectively treat the surface of living mouse
embryos as a
petri dish of cells, and carry out genome wide screens on mouse cells
in their native environment
in vivo,» says Fuchs.
«We couldn't engineer human stomach tissue
in a
petri dish until we first identified how the stomach normally forms
in the
embryo,» explains Wells.
The testing process involved fitting the devices into a standard incubator and using dark field imaging to capture high resolution, single - plane pictures of
embryos housed
in a
petri dish, at five - minute intervals.
Through Zernicka - Goetz's efforts, this process - essential to the formation of natural
embryos - has now for the first time been successfully replicated
in a
petri dish.