The fluorescence emitted by the cells was viewed using a fluorescence microscope, equipped with U-MWB2 optical filters at excitation /
emission wavelengths of 460 ~ 490/520 nm.
Calcein - AM and Hoechst were added at the end of the treatment for 10 minutes and the developed fluorescence was measured in assay buffer in a Victor3 plate reader (Hoechst at excitation and
emission wavelengths of 355 nm and 465 nm, respectively and calcein at excitation and
emission wavelengths of 485 nm and 535 nm, respectively).
The fluorescence emitted by Hoechst 33342 was viewed using a fluorescence microscope, equipped with U-MWU optical filters at excitation /
emission wavelengths of 330-385/420 nm.
Nuclei were stained with Hoechst 33342 (5 μg / ml) and observed by a fluorescence microscope equipped with U-MWU optical filters with a U-MWU optical filter at an excitation wavelength of 355 nm and
an emission wavelength of 420 nm.
Fluorescence intensities were measured at an excitation wavelength of 340 or 380 nm and
an emission wavelength of 510 nm, with a fluorescence spectrometer (Hitachi F - 2500) during stimulation as indicated in Fig. 5.
Not exact matches
Rampadarath explains: «Comparing the VLA images at radio
wavelengths to Chandra's X-ray observations and the hydrogen -
emission detected by Hubble, shows that features are not only connected, but that the radio outflows are in fact the progenitors
of the structures seen by Chandra and Hubble.
The picture which emerges is one
of a twisting jet whose
emission is amplified at different
wavelengths at different times, by the «lighthouse effect.»
The instrument is sensitive to near - infrared light, the
wavelengths at which the
emissions of extremely distant galaxies — stretched by the expansion
of space — shine most brightly.
After being ground up into a fine powder, the substance produced infrared
emissions (phospholescence) with a
wavelength of 900 nm.
And just as we can regard radio
emissions as waves and not as photons because
of their long
wavelength, the gravitational waves that we detected were
of sufficiently long
wavelength that we could indeed regard them as waves.
The night - time
emission of green light, with a
wavelength of 557.7 nanometres, was detected in the Earth's upper atmosphere more than a century ago.
By studying such a large data set — over 200,000 galaxies in 21 different
wavelengths, or colors
of light, from ultraviolet to infrared — astronomers compared the energy
emissions from galaxies across a wide swath
of space and time to read the history
of the universe.
Sky surveys and mappings
of the various
wavelength bands
of electromagnetic radiation (in particular 21 - cm
emission) have yielded much information on the content and character
of the universe's structure.
What is more, because Jupiter's microwave
emissions vary in
wavelength based on the pressure (as well as temperature)
of the atmospheric layers where they originate, observations at multiple
wavelengths allow researchers to create a cross-section through the atmosphere.
Now, in a study published in Nature Nanotechnology on January 11th 2016 (online), a team
of MIT researchers describes another way to recycle light emitted at unwanted infrared
wavelengths while optimizing the
emission at useful visible
wavelengths.
(Because Saturn's atmosphere is largely composed
of hydrogen, aurora
emissions are mainly in the ultraviolet band
of wavelengths.)
The FORTIS spectrographic element splits the light captured by the telescope into segments
of varying
wavelength intensity, which help scientists discern hydrogen
emission and absorption.
But for one lengthy interval during the observations, the team spotted
emissions from hydrogen (at a
wavelength of 121.6 nanometers) in the same region.
Working at the IRAM Plateau De Bure interferometer in the French Alps, the researchers gathered data in the millimetre band, which allows observation
of the
emission from the cold gas which is the primary fuel for star formation and main ingredient
of galaxies, but is almost invisible at other
wavelengths.
The Solar Dynamics Observatory, launched by NASA in February, returns 16 - megapixel images
of the sun on a nearly continuous basis, splits the sun's
emissions into its individual
wavelengths, tracks the propagation
of waves across the sun's surface and maps the ever shifting solar magnetic field.
Beyond the
wavelength coverage
of the space - borne observatories, the spectra taken in Hawaii even turned up a curious new feature — a boost in
emissions of unknown origin at
wavelengths of about 3.3 microns.
To prove their concept
of this multiplex spectral microgel analysis within a microfluidic flow, the team used «different barcodes corresponding to different
emissions at specific
wavelengths and the fluorescence intensity
of known microRNA concentration,» which was measured for calibrations
of the specific microRNA being explored.
They then used a simple microfluidic layout to flow the microgel and allow a continuous measurement
of the fluorescence signal with several
emission wavelengths for the multiplexed barcode detection.
The team determined this by detecting two types
of carbon monoxide signatures, an absorption signature at a
wavelength of about 1.6 micrometers and an
emission signature at about 4.5 micrometers.
Our bodies naturally release heat, in part, through
emission of mid-infrared radiation in the 7 - to 14 - µm
wavelength range.
The
emission wavelength used for cell sorting
of DiI - Ac - LDL - labeled cells was 550 nm.
«Nonlinear stage - scanning confocal microscopy is critical because it allows us to rapidly measure the nonlinear
emission from thousands
of different nanostructures while minimizing the potential systematic errors, such as intensity or beam pointing variations, often associated with tuning the
wavelength of an ultrafast laser,» O'Brien says.
Illuminating the cell with a laser results in the
emission of photons with a
wavelength in the infrared spectrum range.
Los Alamos National Laboratory has produced the first known material capable
of single - photon
emission at room temperature and at telecommunications
wavelengths.
This might indicate that smaller (and warmer) dust grains are responsible for the 100 μm
emission than at the longer
wavelengths, in agreement with the theoretical predictions
of van Marle et al. (2011).
Los Alamos National Laboratory researchers have produced the first known material capable
of single - photon
emission at room temperature and at telecommunications
wavelengths, using chemically functionalized carbon nanotubes.
Those bands were not seen again until 2011 when the the team observed the planet with Keck Observatory's NIRSPEC, a unique, near - infrared spectrograph that combines broad
wavelength coverage with high spectral resolution, allowing the observers to clearly see subtle
emissions from the bright parts
of Saturn.
At millimetre
wavelengths emission from the CO molecule allows astronomers to obtain high - resolution maps
of the gas
emission from the strong stellar wind generated by the AGB stars.
Quantum dots are nanoparticles made
of a semiconductor material, that have unique optical properties: the
wavelength (and thus color)
of their light
emission depends on their size rather than the material they are made
of.
Ongoing radio observations (SMA, JCMT, VLA)
of Sirius A are being used to set an observationally determined standard for stellar atmosphere modeling and debris disk studies around A stars, as well as to take the first step toward characterizing potential intrinsic uncertainty in stellar
emission at these
wavelengths.
Webb's ambitious design tackles the two main challenges for an infrared telescope: it has to have a large mirror in order to best capture long infrared
wavelengths; it also has to be kept cold, in order to keep unwanted sources
of infrared light from interfering with the
emissions it attempts to detect.
The practical brightness takes all application specific parameters into account, including the specs
of your microscope (excitation
wavelength, available
emission filters, and detector sensitivity) and the biological system (temperature, prokaryote versus eukaryote, background fluorescence).
Natural astronomical masers — the analog
of laser
emission at microwave
wavelengths — are one class
of coherent sources, but these emit in specific
wavelengths.
The European X-ray Observatory Satellite (EXOSAT), developed by the European Space Agency, was capable
of greater spectral resolution than the Einstein Observatory and was more sensitive to X-ray
emissions at shorter
wavelengths.
A number
of similar black hole exploration methods have also been proposed in optical / infrared spectra so far, but one crucial problem is that
emissions at these
wavelengths are absorbed by interstellar dust particles although the more active black holes contain more dust particles.
Dronpa is photoactivated by blue
wavelengths of light, causing
emission of green fluorescence, but then further exposure to blue light also inactivates the chromophore, leading to a non-fluorescent state.
That first quasar and others identified later puzzled astronomers because, when their light was analyzed to find the characteristic «signature»
of emission at specific
wavelengths shown by particular atoms, the pattern was at first indecipherable.
If such observations go forward, the telescope will provide an unparalleled view
of Proxima b. JWST is optimized for infrared
wavelengths, which can be used to examine a planet's heat
emissions.
They observed L1551 NE in the
emission from dust at a 0.9 - mm
wavelength, a tracer
of distribution
of interstellar materials, and carbon monoxide molecular
emission, which can be used to study gas motion with the Doppler Effect.
The goal
of the research group is to establish a new exploration method using as reference various molecular / atomic
emission lines which can be observed at millimeter / submillimeter
wavelengths (* 3).
These longer -
wavelength observations helped to measure the properties
of clouds in the planet's atmosphere that absorb and re-radiate infrared
emission.»
LOS ALAMOS, N.M., July 31, 2017 — Los Alamos National Laboratory has produced the first known material capable
of single - photon
emission at room temperature and at telecommunications
wavelengths.
Image recording was performed with an excitation
wavelength of 633 nm to scan the respective
emission profiles and recording the
emission in 10 nm windows from 660 to 780 nm.
A specifically tailored Uppsala model based on the MARCS code and extending further in
wavelength is used to gauge the
emission characteristics
of alpha Cen A in the FIR.
Intensity as recorded in zebrafish (crosshairs in Fig. 4a show where measurement took place) at an excitation
wavelength of 633 nm and scanned for
emission at 11 nm intervals from 654 nm to 794 nm.