Sentences with phrase «emissions filters»

The methylation reactions were followed by monitoring the increase in fluorescence using BioTek Synergy2 plate reader with 360/40 nm excitation filter and 528/20 nm emission filter for 20 min in 384 - well format.

The practical brightness takes all application specific parameters into account, including the specs of your microscope (excitation wavelength, available emission filters, and detector sensitivity) and the biological system (temperature, prokaryote versus eukaryote, background fluorescence).

The practical brightness assay (employing co-expressing fluorescent proteins or using endogenous tagged genes) can be used to verify performance under different imaging conditions (e.g. different emission filters) or different set - ups.

The simplest and the most popular one is the sensitised emission method, where the donor is excited by a specific wavelength of light and the signal is collected by using emission filters chosen for the donor fluorescence and the acceptor fluorescence.

The sample was injected with live cell nucleic acid stain [STYO 13] which is taken up by live bacteria and shows as bright green when viewed using a 500 nm - long pass emission filter for fluorescence detection.

Excitation and emission filters for the SYPRO - Orange dye were set to 465 nm and 590 nm, respectively.

After the light passes through the sample and the objective lens, an emission filter blocks all light except for that emitted by the green fluorescent dye specific to the TB bacilli.

First, the tubes were imaged in reflectance mode against a dark background for a series of excitation wavelengths and emission filters to establish a baseline of fluorescent protein expression.