Finding Detours Around Traffic Jams: Characterization of Clathrin - Independent
Endocytosis in Yeast, speaker Dr. Derek Prosser
A long - standing goal of our group is to elucidate the role of
endocytosis in cell signalling and cancer.
Clathrin - mediated
endocytosis in vivo.
Endocytosis in a human stem cell derived organoid.
In one project, we study the complex and dynamic protein machinery that performs clathrin - mediated
endocytosis in yeast.
Applications of pHluorin for Quantitative, Kinetic and High - throughput Analysis of
Endocytosis in Budding Yeast.
We have been studying
endocytosis in a broader, systems perspective, by combining quantitative imaging, genome - wide RNAi screens with systems biology analysis.
Clathrin is Important for Normal Actin Dynamics and Progression of Sla2p - Containing Patches During
Endocytosis in Yeast.
Clathrin - mediated
endocytosis in snake motor terminals is directly facilitated by intracellular Ca2 +.
Altered cortical GABAA receptor composition, physiology, and
endocytosis in a mouse model of a human genetic absence epilepsy syndrome.
Electrogenic NBCe1 (SLC4A4), but not electroneutral NBCn1 (SLC4A7), cotransporter undergoes cholinergic - stimulated
endocytosis in salivary ParC5 cells.
«If you inhibit [
endocytosis in the nerve terminal], then the vesicle recycling becomes slower and the supply of the vesicles is inhibited,» OIST Professor Tomoyuki Takahashi from the Cellular and Molecular Synaptic Function Unit explains.
The UT Southwestern researchers monitored
endocytosis in NSCLCs and found significant heterogeneity among the various cell lines, underlining the astounding variety and personal nature of cancer types.
A team at Warwick Medical School, University of Warwick, has managed to trigger clathrin - mediated
endocytosis in the lab.
While working on his Ph.D., Dr. Taraska investigated the processes of triggered exocytosis and
endocytosis in neuroendocrine cells with high - resolution microscopy methods.
«Plants recycle, too: Biologists have now identified a new protein complex which is crucial for
endocytosis in plants.»
Not exact matches
Endocytosis of Frizzled 4 (Fz4)
in human embryonic kidney 293 cells was dependent on added Wnt5A protein and was accomplished by the multifunctional adaptor protein β - arrestin 2 (βarr2), which was recruited to Fz4 by binding to phosphorylated Dvl2.
Wnt proteins, regulators of development
in many organisms, bind to seven transmembrane — spanning (7TMS) receptors called frizzleds, thereby recruiting the cytoplasmic molecule dishevelled (Dvl) to the plasma membrane.Frizzled - mediated
endocytosis of Wg (a Drosophila Wnt protein) and lysosomal degradation may regulate the formation of morphogen gradients.
These findings provide a previously unrecognized mechanism for receptor recruitment of β - arrestin and demonstrate that Dvl plays an important role
in the
endocytosis of frizzled, as well as
in promoting signaling.
Among all the genes that control
endocytosis, the authors of the study focused specifically on one, called RAB7; this gene is highly expressed
in melanoma cells.
While proteins involved
in endocytosis, such as clathrin, are conserved across the animal and plant kingdoms, the members of the TPLATE complex appears specially designed for plants.
Using state - of - the - art techniques, researchers of VIB, Ghent University and the Max - Planck - Institute
in Potsdam - Golm have now identified an adaptor protein complex which is essential for
endocytosis and which only exists
in plants.
The latest frontier
in this search for cancer telltales is the cellular process of
endocytosis, which is the way cells take outside materials like proteins inside.
Researchers from the Mechanobiology Institute, Singapore (MBI) at the National University of Singapore, report that
endocytosis, which was previously thought to be a random process, actually occurs
in a coordinated manner through collective dynamics.
But by hierarchical clustering of observed alterations
in endocytosis, the researchers identified two distinct cancer phenotype clusters, one marked by mutations
in the oncogene KRAS
in mesenchymal cells and the other by changes
in epithelial cells.
«Non-small cell lung cancers can be sorted
in clusters by endocytic changes: Sorting cancer by defective
endocytosis genes.»
They did it by using a chemical rapamycin which diffuses
in to the cell allowing them to trigger
endocytosis to occur all over the cell.
Whereas the chemical across the cell the light can be focused
in a narrow region causing
endocytosis to be triggered only
in that region.
Endocytosis is not normal
in cancer cells but how dysregulated the process is
in cancer cells has just been revealed by Sarah Elkin and colleagues
in the University of Texas (UT) Southwestern Medical Center lab of Sandra Schmid.
In particular, this study demonstrates the capability to track structural dynamics and remodeling of the cell surface, such as morphogenesis of filopodia, membrane ruffles, pit formation or endocytosis, in response to environmental stimulant
In particular, this study demonstrates the capability to track structural dynamics and remodeling of the cell surface, such as morphogenesis of filopodia, membrane ruffles, pit formation or
endocytosis,
in response to environmental stimulant
in response to environmental stimulants.
Apparently, too much alpha - synuclein
in the nerve terminal causes microtubules to over-assemble and somehow get
in the way of
endocytosis.
However, when they are not
in an eisosome, these proteins are quickly absorbed again through
endocytosis.»
We know the initial target and the mechanism... [But]
in order to step into [the treatment] stage, we should probably work a little bit more to know by what mechanism the microtubules interfere with
endocytosis.»
Researchers at the Okinawa Institute of Science and Technology Graduate University (OIST) have found that the protein hinders a key step involved
in the transmission of neuronal signals, which is essential for higher - brain functioning: vesicle
endocytosis at the nerve terminal.
RNA interference screen reveals an essential role of Nedd4 — 2
in dopamine transporter ubiquitination and
endocytosis.
Use of dynasore, the small molecule inhibitor of dynamin,
in the regulation of
endocytosis.
Three ubiquitin conjugation sites
in the amino terminus of the dopamine transporter mediate protein kinase C — dependent
endocytosis of the transporter.
A Second Las17 Monomeric Actin - Binding Motif Functions
in Arp2 / 3 - Dependent Actin Polymerization During
Endocytosis.
Visualization of Rab5 activity
in living cells by FRET microscopy and influence of plasma - membrane - targeted Rab5 on clathrin - dependent
endocytosis.
Endocytosis regulates cell soma translocation and the distribution of adhesion proteins
in migrating neurons.
Regulation of clathrin adaptor function
in endocytosis: novel role for the SAM domain.
Roles of AP - 2
in clathrin - mediated
endocytosis.
Inactivation of Tor proteins affects the dynamics of endocytic proteins
in early stage of
endocytosis.
We have shown that the ubiquitous clathrin heavy chain (CHC), well characterized for its role
in intracellular membrane traffic and
endocytosis from the plasma membrane (PM), forms large plaques connected to α - actinin and actin filaments.
Mild
in vitro trauma induces rapid Glur2
endocytosis, robustly augments calcium permeability and enhances susceptibility to secondary excitotoxic insult
in cultured Purkinje cells.
Endocytosis is the process
in which a part of a cell engulfs external molecules for further processing.
In the living state, the cells undergo characteristic movements, and unlike macrophages, do not appear to engage in active endocytosi
In the living state, the cells undergo characteristic movements, and unlike macrophages, do not appear to engage
in active endocytosi
in active
endocytosis.
The Johannes group has made two major contributions
in this context: the discovery of a membrane trafficking interface between early endosomes and the Golgi apparatus, and the demonstration that dynamic protein - induced glycosphingolipid reorganization acts as a driving force for membrane invagination
in clathrin - independent
endocytosis.
In the Endocytic Trafficking and Intracellular Delivery team we study the cell biology of carbohydrate - binding proteins (lectins) at the level of cellular entry though clathrin - independent
endocytosis, retrograde sorting on early endosomes, and translocation to the cytosol.
We are also investigating
endocytosis of the cell surface receptor, Notch, a protein involved
in cell fate specification (6).