Sentences with phrase «endoderm differentiation»

Analysis of Oct4 protein expression in differentiated Ara - C cultures (DACs) showed a slight increase of Oct4 protein by day four of aggregate formation (Figure 1C), consistent with previous reports examining Oct4 mRNA dynamics [22] and the finding that primitive endoderm differentiation is accompanied by a transient increase in Oct4 protein levels [23].

HV clones were differentiated in aggregation culture in the presence of activin under conditions designed to promote anterior endoderm differentiation and Hex expression.

We show that the fraction of cells present within this state is influenced by factors that both promote and suppress primitive endoderm differentiation, but conditions that support ES cell self - renewal prevent their progression into differentiation and support an equilibrium between this state and at least one other that resembles the Nanog positive inner cell mass of the mammalian blastocysts.

Rational Bioprocess Design for Human Pluripotent Stem Cell Expansion and Endoderm Differentiation Based on Cellular Dynamics.

For definitive endoderm differentiation, spontaneously formed EBs on day 6 were plated onto matrigel - coated dishes and maintained in a low concentration of fetal bovine serum (FBS) in combination with a high concentration of recombinant Activin A. Scale - like cells appeared (Figure 5A), which were positive for definitive endoderm markers such as Foxa2, Sox17 and Gata4.

«The most important application of this patent will be the use of our patented human endoderm cells to screen for agents that potentially affect endoderm differentiation, for example, to any of the important cells derived from endoderm such as lung, liver, intestine, pancreas, thymus, parathyroid and thyroid.»

Our technology enables the identification of potential endoderm differentiation factors, leading to potential discovery of new drugs & agents for research, and, ultimately, therapeutic use.»

Not exact matches

Professor Heiko Lickert, director of the IDR, in collaboration with Professor Gunnar Schotta of LMU München, showed that the Wnt / β - catenin signaling pathway regulates Sox17, which in turn regulates molecular programs that assign pluripotent cells to the endoderm, thus inducing an initial differentiation of the stem cells.

The portfolio includes U.S. and foreign patents and applications directed to culture, expansion and scale - up of pluripotent stem cells and cell types derived therefrom, various differentiation processes for production of mesendoderm, definitive endoderm, PEC - 01, and endocrine precursors, as well as claims directed to the Company's Encaptra ® macro-encapsulation device platform.

Novocell scientists pioneered a differentiation process that successfully engineers hES cells into definitive and pancreatic endoderm cells necessary for pancreas formation.

The ability of Nanog to suppress early Hex positive endoderm states is consistent with both the mutually exclusive nature of Nanog and Gata6 expression in vivo [17] and the ability of Nanog to suppress Gata6 positive PrEn differentiation, in vitro [39].

J, Hematoxylin and eosin staining of teratoma sections generated from integration - free iPSC lines showing differentiation in three germ layers: goblet cells in gastro - intestinal (GI) tract (endoderm); neural rosettes (ectoderm) and blood vessels, muscle and cartilage / bone (mesoderm).

ViaCyte's patent portfolio covers many of the intermediary cell types generated when human pluripotent stem cells progress along the differentiation pathway to pancreatic progenitor cells, including mesendoderm and definitive endoderm.

Moreover, ES cell differentiation in vitro recapitulates events that take place during early embryonic development including the formation of the three germ layers of ectoderm, mesoderm and endoderm, and the emergence of endothelial, hematopoietic, cardiac, neuronal and hepatic or pancreatic cells [8], [9].

Differentiation into extraembryonic endoderm [21] or neural progenitors [33] are frequent early outcomes of spontaneous differentiation when human ES cells are cultured in the presence of a feeder cell layer, and it is interesting to speculate that the cells are being primed fDifferentiation into extraembryonic endoderm [21] or neural progenitors [33] are frequent early outcomes of spontaneous differentiation when human ES cells are cultured in the presence of a feeder cell layer, and it is interesting to speculate that the cells are being primed fdifferentiation when human ES cells are cultured in the presence of a feeder cell layer, and it is interesting to speculate that the cells are being primed for these fates.

We have previously reported that our 26 - d protocol closely recapitulates key stages of biliary development, starting with the differentiation of hPSCs into endoderm and subsequently into foregut progenitor (FP) cells, followed by the generation of hepatoblasts (HBs), cholangiocyte progenitors (CPs) expressing early biliary markers and mature CLCs displaying cholangiocyte functionality.

«Novocell is a leader in differentiation of stem cells to create functional islet cells of the pancreas, and this broad patent significantly enhances our proprietary position and further secures our investment in the development of a diabetes cell therapy and potentially other endoderm - derived cell products,» said Edward Baetge, Ph.D., Senior Vice President and Chief Scientific Officer of Novocell.

It is probable that these cells represent extraembryonic endoderm, and that they arise directly from stem cells primed to differentiation into this lineage.

The next day, the resulting cells were grown in CDM - PVA supplemented with Ly294002 (10 μM; Calbiochem), Activin (100 ng / ml); FGF2 (40 ng / ml; R&D Systems), and BMP4 (10 ng / ml; R&D Systems) to drive their differentiation toward definitive endoderm.