Sentences with phrase «endoderm lineages»

The epiblast is the source of all embryonic tissue and the PrEn the source of both extra-embryonic endoderm lineages, visceral and parietal.

To induce mesoderm and endoderm lineages from iPS cells, cells were grown as embryoid bodies (EB) in DMEM / F12 with 10 % fetal bovine serum for 2 weeks.

On the other hand, cells of line 4.1 differentiated into endoderm lineages more easily than cells of line 11.1 (data not shown).

(A) A low concentration of FBS in combination with a high concentration of recombinant Activin A could induce iPS cells of line 11.1 into a definitive endoderm lineage.

Here we use a transgenic allele designed to detect low level transcription in the primitive endoderm lineage as a tool to identify an immediate early endoderm - like ES cell state.

A more recent study showed overlapping expression of nanog with GATA - 6 and a Pdgfra reporter, markers of the primitive endoderm lineage, from the morula to the 64 cell stages [8], suggesting a gradual transition from a pluripotency program to a committed state.

San Diego, CA (March 31, 2009)-- Novocell, Inc., a stem cell engineering company, today announced that it has received U.S. Patent 7,510,876 with claims covering human definitive endoderm cells, an essential cell for generating not only pancreatic type cells, which Novocell is developing for use as a cell therapy for diabetes, but also other endoderm lineage - derived tissues and organs such as lungs, intestine, liver, thymus and thyroid.

«This composition patent is a milestone achievement for Novocell and is the culmination of extensive research that opened the door to the endoderm lineage,» said Fred Middleton, Chairman & Acting CEO of Novocell.

Not exact matches

When the ESCs had no Chd1 at all, the cells could not differentiate into all of the three germ layers (specifically, endoderm was not detected, and a preference was found for neuronal lineages).

Simultaneously, expression of markers for major developmental lineages, including trophectoderm (Cdx2), ectoderm (Fgf5), mesoderm (T) and endoderm (Gsc, Sox7 and Afp), was activated (Figure 4B, bottom panel).

However, cells derived from the early epiblast are only able to contribute to embryonic lineages and not those derived from the PrEn [18]--[20], while PrEn cells can only contribute to their own lineage by colonizing the visceral and mostly parietal endoderm in chimera experiments [20]--[22].

In support of this idea, Beddington and Robertson originally observed ES cell contribution to all the extra-embryonic lineages, but in particular to parietal endoderm [23].

The Venus positive population does not efficiently contribute to somatic lineages, appears at the outside of EBs, and has the capacity to colonize the visceral and parietal endoderm in chimeras.

However, when challenged to differentiate immediately after purification, each exhibits strong lineage bias, with the endoderm marker - expressing fraction unexpectedly able to contribute to the extra-embryonic endoderm in chimeric embryos.

On the other hand, ES cells and cells differentiating into endoderm and mesoderm lineages from ES cells express OCT - 4 in mice and humans [66], and SSEA1 expression is found in other type of cells including neural stem cells and mesenchymal stem cells [67], [68].

Mouse embryonic stem cells derived from the epiblast contribute to the somatic lineages and the germline but are excluded from the extra-embryonic tissues that are derived from the trophectoderm and the primitive endoderm upon reintroduction to the blastocyst.

It is probable that these cells represent extraembryonic endoderm, and that they arise directly from stem cells primed to differentiation into this lineage.