Sentences with phrase «endoderm shown»

(A) iPS cells can form embryoid bodies (EB) and can differentiate in vitro to endoderm shown by alpha fetoprotein (AFP)(B), mesoderm shown by smooth muscle actin (SMA) and desmin (D, E), and ectoderm shown by GFAP and SMI31 staining (G, H).

Not exact matches

«Through studies like that, we've been able to show how the main so - called germ layers for the embryo are formed — ectoderm, mesoderm, and endoderm.

Professor Heiko Lickert, director of the IDR, in collaboration with Professor Gunnar Schotta of LMU München, showed that the Wnt / β - catenin signaling pathway regulates Sox17, which in turn regulates molecular programs that assign pluripotent cells to the endoderm, thus inducing an initial differentiation of the stem cells.

But then Stainier and colleagues showed that the endoderm was also somehow responsible for the migration of the heart cell buds in the layer of cells above it, known as the mesoderm.

As shown in Figure 4, all three germ layers developed in our cultures as shown by alphafetoprotein (AFP) for endoderm (Figure 4B), smooth muscle actin (SMA) and desmin for mesoderm (Figure 4D, E), glial fibrillary acid protein (GFAP) and SMI31 for ectoderm (Figure 4G, H).

On the other hand, cells of line 4.1 differentiated into endoderm lineages more easily than cells of line 11.1 (data not shown).

Black arrowheads show the presence of LacZ positive cells in the parietal endoderm.

Black arrows show the presence of LacZ positive cells in the visceral endoderm.

(D) Sections of EBs grown under the same conditions as in part (C), showing that the outer layer consists of visceral endoderm as marked by Gata6, FoxA2, and Sox17 immunostaining (shown as red).

J, Hematoxylin and eosin staining of teratoma sections generated from integration - free iPSC lines showing differentiation in three germ layers: goblet cells in gastro - intestinal (GI) tract (endoderm); neural rosettes (ectoderm) and blood vessels, muscle and cartilage / bone (mesoderm).

Moreover, as it has recently been shown that VE can contribute to the embryonic gut [55], the distinction between visceral and definitive endoderm begins to blur and the inability of ES cells to contribute to the VE becomes more puzzling.

We show that the fraction of cells present within this state is influenced by factors that both promote and suppress primitive endoderm differentiation, but conditions that support ES cell self - renewal prevent their progression into differentiation and support an equilibrium between this state and at least one other that resembles the Nanog positive inner cell mass of the mammalian blastocysts.

A more recent study showed overlapping expression of nanog with GATA - 6 and a Pdgfra reporter, markers of the primitive endoderm lineage, from the morula to the 64 cell stages [8], suggesting a gradual transition from a pluripotency program to a committed state.

Analysis of Oct4 protein expression in differentiated Ara - C cultures (DACs) showed a slight increase of Oct4 protein by day four of aggregate formation (Figure 1C), consistent with previous reports examining Oct4 mRNA dynamics [22] and the finding that primitive endoderm differentiation is accompanied by a transient increase in Oct4 protein levels [23].