Sentences with phrase «endogenous epitope tags»
Because of these limitations, the AAV approach is best suited for tissue culture based approaches that require modification of only a single allele of a gene, such as the introduction of heterozygous endogenous epitope tags, or the introduction of dominant mutations of a gene into the endogenous allele in cultured cells.
http://blog.addgene.org/ Not exact matches
Some examples from their lab include using AAV to introduce epitope tags into the endogenous alleles of the p53 and PTEN tumor suppressor genes in human cells (Kim et al 2008).
Kim, Jung - Sik, et al. «Epitope tagging of endogenous genes in diverse human cell lines.»