Furthermore, data from RT - PCR analysis showed that transgenic 4 factors were completely silenced and
endogenous pluripotency markers Oct4, Sox2, Rex1 as well as Nanog were successfully activated (Figure 3C).
(C) RT - PCR results using specific primers against transgenic 4 factors and
endogenous pluripotency markers.
RT - PCR analysis of RNA samples from EBs on days 3, 6 and 9 revealed a gradual decrease in expression of
endogenous pluripotency markers such as Nanog, Rex1, Oct4 and Sox2 as compared to their expression in undifferentiated iPS cells (Figure 4B, upper panel).