Sentences with phrase «endonuclease contains»

Gesicle loading utilizes the iDimerize system, which enables inducible protein - protein interactions: The membrane - bound CherryPicker red fluorescent protein on the surface of the gesicle is tagged with one dimerization domain, and the Cas9 endonuclease contains another dimerization domain.

Not exact matches

In their zebrafish model, the barcode contains 10 20 - base - pair sequences targeted by Cas9 endonuclease, with each of the sequences matching a specific single guide RNA.

The kit includes lyophilized Xfect Transfection Reagent and gesicle packaging mixes which contain coding sequences for the nanovesicle - inducing glycoprotein, Cas9 endonuclease, and CherryPicker red fluorescent protein.

Then, using the CRISPR / Cas9 endonuclease system and a guide RNA targeting the region containing the SCD mutation, the group successfully corrected one mutated HBB allele with no undesired insertions of the donor vector seen elsewhere in the genome.