Sentences with phrase «endothelial no»

Endothelial cells plated onto two - chamber slides at a density of 2 × 105 cells / well in 10 % DMEM were incubated overnight.

Thus, our selection strategies, which were based on targeting endothelial populations that expressed both of these inducible glycoproteins, suggest that the endothelial cell lines generated in this study are most likely from this microcirculatory region.

These results suggest that response to inflammatory cytokines by endothelial cells is not required for NS1 to cause blood vessel leakage.

As expected, all of the endothelial cell lines showed diminished constitutive expression of E-selectin (data not shown) and VCAM - 1 (Fig. 4C) ⇓ in comparison with surface levels of ICAM - 1 (Fig. 4B) ⇓.

We have observed on several occasions that, for the majority of endothelial cell lines, the transfer to 37 °C conditions for 72 h correlated with a 50 % reduction in cell proliferation, a value that continued to decrease with time.

Phase - contrast images of organ - derived endothelial cells (37 °C culture conditions) that had undergone two successive selection processes by flow cytometry.

This is the first evidence that starvation of endothelial cells could offer new therapeutic opportunities for the treatment of excessive angiogenesis in diseases (like cancer).

One of the primary objectives of this study was to establish a protocol to facilitate the identification and selection of microvascular endothelial cells from primary organ cultures with a high degree of precision.

A marker in bile known as vascular endothelial growth factor (VEGF) plays an important role in the growth of cancerous tumors according to new research presented at the 78th Annual Scientific Meeting of the American College of Gastroenterology.

Peter Carmeliet and his team demonstrated that endothelial cells can be paralyzed by blocking glycolysis and consequently stop to form blood vessels.

The selection strategy targeted cell populations expressing the inducible endothelial cell adhesion molecules, E-selectin and VCAM - 1, and proved successful in generating microvascular endothelial cell lines from a number of different organs.

When primary cultures from these mice are stimulated with proinflammatory cytokines, the endothelial cell fraction responds by up - regulating the inducible endothelial cell adhesion molecules, E-selectin and VCAM - 1.

At that key point, Takebe added two more types of cell known to help to recreate organ - like function in animals: endothelial cells, which line blood vessels, taken from an umbilical cord; and mesenchymal cells, which can differentiate into bone, cartilage or fat, taken from bone marrow.

The endothelial cell fraction was labeled by resuspending the pellet in 2 % DMEM containing 4 μg / ml PE - conjugated rat antimouse E-selectin mAb and 2 μg / ml FITC - conjugated rat antimouse VCAM - 1 mAb.

Phase - contrast images of organ - derived endothelial cells (37 °C) on Matrigel surface (A) and after 4 h of incubation with DiI - Ac - LDL (B).

The highest level of expression was found in heart and bladder endothelial cells.

Similar to cells growing at 33 °C, endothelial cells plated in the 37 °C environment also engaged in cell division during the first 24 h. However, during the next 72 h, the growth of most endothelial cells in 37 °C conditions dropped to 50 % of that of their counterparts growing at 33 °C (data not shown).

Each of the endothelial cell lines established from the H - 2Kb - tsA58 transgenic mice was capable of generating vascular - like channels on Matrigel within 12 h.

As shown in Fig. 4B, suppressive ability of miR - 7 on both transmigration through endothelial cells and Matrigel was significantly attenuated by ectopic expression of KLF4, suggesting that miR - 7 suppresses invasive ability of CSCs by blocking KLF4 expression.

Matrigel - containing plates were allowed to incubate at 37 °C for 30 min and then seeded with endothelial cells at a density of 4 × 104 cells / well in 10 % DMEM.

The constitutive expression of these endothelial cell adhesion molecules was very similar for most of the lines derived from different organs, i.e., very low expression levels of E-selectin and VCAM - 1 and more pronounced expression of ICAM - 1.

Specifically, they were able to determine that Wars2 plays an important role in supplying sufficient endothelial cells, the building blocks of blood vessels, for angiogenesis.

The constitutive and inducible expression of the endothelial cell adhesion molecules E-selectin, ICAM - 1, and VCAM - 1 was assessed by immunohistochemistry (Figs. 4, A — C) ⇓.

↵ 3 The abbreviations used are: HUVEC, human umbilical vein endothelial cell; DiI - Ac - LDL, 1,1 ′ - dioctadecyl - 3,3,3 ′, 3 ′ - tetramethyl - indocarbocyanine acetylated low - density lipoprotein; FACS, fluorescence - activated cell sorting; FGFR, fibroblast growth factor receptor; Flk, fetal liver kinase; ICAM, intercellular adhesion molecule; mAb, monoclonal antibody; PE, phycoerythrin; TNF, tumor necrosis factor; VCAM, vascular cell adhesion molecule.

A, comparison of E-selectin expression in control colon (top left panel) to TNF - α - stimulated expression in colon (center panel) and ovarian (right panel) microvascular endothelial cells.

Tissue - specific microvascular endothelial cell lines from H - 2K (b)- tsA58 mice for studies of angiogenesis and metastasis

We capitalized on one of the innate responses of microvascular endothelial cells, i.e., the up - regulation of cell adhesion molecules in response to inflammatory agents, to generate endothelial cell lines from different organs.

The ability of the endothelial cells to form capillary - like structures was assessed by placing them on the solubilized basement membrane preparation Matrigel (Becton Dickinson, San Jose, CA), according to the manufacturer's instructions.

One of the major impediments to obtaining a large number of endothelial cells from different tissues has been the inability to purify and propagate these cells in culture.

The rate of cell proliferation varied only slightly between the different endothelial cell lines (Fig. 3) ⇓.

Murine endothelioma cells that express several endothelial markers (Ref.

Endothelial cells (passage 5) from each line were plated onto 96 - well plates at a density of 1000 cells / well in 10 % DMEM.

B, CSCs isolated from 231BrM infected with lentivirus of miR7 or KLF4 expression plasmids were seeded on top of mouse brain endothelial cells or Matrigel.

This latter observation adds to the expanding body of evidence indicating that brain microvascular endothelial cells lack receptors for chemically modified LDL (27, 28).

Cultured endothelial cells from lung, colon, kidney, bladder, liver, and ovary displayed a cobblestone appearance after reaching confluence.

To overcome these limitations, we used two - color flow cytometry to identify and select microvascular endothelial cells from primary cultures obtained from different organs of mice whose tissues harbor a temperature - sensitive SV40 large T antigen (H - 2Kb - tsA58 mice; ImmortoMice).

This experiment uses quantitative PCR to detect the expression level of POSTN in CD34 + / CD31 − pulmonary fibroblasts, CD31 + endothelial cells, and CD45 + immune cells isolated from lungs of mice with macrometastases, which is a replication of the experiment reported in Figure 2H.

High Serum Vascular Endothelial Growth Factor (VEGF) Level Is An Adverse Prognostic Factor In High Risk Diffuse Large B - Cell Lymphoma (DLBCL) Patients Treated with Dose - Dense Chemoimmunotherapy and Systemic CNS Prophylaxis.

The anti-vascular endothelial growth factor drugs ranibizumab and aflibercept, used to treat vision loss from diabetic macular edema (DME), and approximately 20 to 30 times more expensive than bevacizumab, are not cost - effective for treatment of DME compared to bevacizumab unless their prices decrease substantially, according to a study published online by JAMA Ophthalmology.

TNF - alpha and VEGF have proven to be particularly effective: TNF - alpha allows adhesion to endothelial cells, while VEGF authorizes the passage through the endothelial barrier.

The vessels are lined with endothelial cells, which secrete chemical signals that help stem cell survive.

Dengue virus NS1 triggers endothelial permeability and vascular leak, and vaccination with NS1 induces a protective antibody response.

Next, endothelial primary cells (EPCs) were grown on one side of the silk scaffold and megakaryocytes were seeded on the other side.

Researchers knew at the time that tumors can induce normal endothelial cells to form new blood vessels, a process called angiogenesis.

To begin, the researchers knew they would need a structure that could support endothelial cells, ECMs, and megakaryocytes, without adversely activating the cells or platelets.

It took advantage of the brain's own mechanism for getting a necessary nutrient, iron, across the lining of endothelial cells that form the blood - brain barrier.

We also demonstrate that NS1 from DENV1, DENV2, DENV3, and DENV4 triggers endothelial barrier dysfunction, causing increased permeability of human endothelial cell monolayers in vitro.

Genentech scientists led by Mark Dennis designed an antibody with two arms — one that binds to BACE1 and inhibits its activity and one that binds to the transferrin receptor and tricks endothelial cells into transporting the antibody across the blood - brain barrier.

However, no viral proteins have been directly implicated in triggering endothelial permeability, which results in vascular leakage.

When a new vessel is needed, the endothelial cells direct the process by allowing the recruitment of blood cells required for neovascularization, commonly called angiogenesis.