Sentences with phrase «endothelial cell lines»

Oversaw Dosimetry accountability, and successfully maintained cancer and endothelial cell lines.

The immortalized endothelial cell lines established from H - 2Kb - tsA58 mice provide, for the first time, a cell culture system to examine factors regulating angiogenesis and tumor cell arrest in different organ systems.

The onset of tube structure formation could be visualized as early as 4 h after plating for most endothelial cell lines, with extensive networks becoming visible within 12 h after plating.

Many of the endothelial cell lines described here have been considered previously inaccessible for study of tumor angiogenesis, progression, and metastasis.

The resulting endothelial cell lines described in this study retain their phenotype after repeated passage, require minimal nutritional support and, importantly, may be useful for identifying factors that regulate endothelial cell proliferation and tumor cell adhesion in different anatomical regions.

However, efforts to examine the in vitro behavior of tumor cells with endothelial cells from different anatomical regions have been prohibited because, in large part, of the limited number of organ - derived endothelial cell lines available for study.

However, unlike HUVECs or other commercially available endothelial cell lines, the endothelial cells generated from the H - 2Kb - tsA58 mice retained their ability to mobilize these adhesion molecules despite undergoing numerous population doublings (currently 30 passages).

Three types of structural variations were visible in the tissue - specific endothelial cell lines (Fig. 2) ⇓.

Each of the endothelial cell lines demonstrated an ability to orient into capillary - like structures when placed onto a Matrigel surface (Fig. 5A) ⇓.

Thus, our selection strategies, which were based on targeting endothelial populations that expressed both of these inducible glycoproteins, suggest that the endothelial cell lines generated in this study are most likely from this microcirculatory region.

As expected, all of the endothelial cell lines showed diminished constitutive expression of E-selectin (data not shown) and VCAM - 1 (Fig. 4C) ⇓ in comparison with surface levels of ICAM - 1 (Fig. 4B) ⇓.

We have observed on several occasions that, for the majority of endothelial cell lines, the transfer to 37 °C conditions for 72 h correlated with a 50 % reduction in cell proliferation, a value that continued to decrease with time.

The selection strategy targeted cell populations expressing the inducible endothelial cell adhesion molecules, E-selectin and VCAM - 1, and proved successful in generating microvascular endothelial cell lines from a number of different organs.

Each of the endothelial cell lines established from the H - 2Kb - tsA58 transgenic mice was capable of generating vascular - like channels on Matrigel within 12 h.

Tissue - specific microvascular endothelial cell lines from H - 2K (b)- tsA58 mice for studies of angiogenesis and metastasis

We capitalized on one of the innate responses of microvascular endothelial cells, i.e., the up - regulation of cell adhesion molecules in response to inflammatory agents, to generate endothelial cell lines from different organs.

The rate of cell proliferation varied only slightly between the different endothelial cell lines (Fig. 3) ⇓.

Epithelial cells form tissue layers that cover our skin and the inner surfaces of most of our internal organs, while endothelial cells line the adjacent blood - transporting vessels and capillaries that support their functions.

Luster's team hopes to further investigate the characteristics of joints that underlie the critical role of C5a in initiating type III hypersensitivity and whether specific molecules expressed on endothelial cells lining joints play a role in transporting C5a and chemokines from the joint space into adjacent blood vessels.

Current thinking regarding type III hypersensitivity is that immune cells within tissues sense the presence of these immune complexes (ICs) through specific receptor molecules and release inflammatory factors called cytokines that activate the endothelial cells lining adjacent blood vessels to promote the recruitment of neutrophils.

A specific lung - targeting molecule is attached to the carrier's surface and the steriod, dexamethasone, an anti-inflammatory, is loaded into the nanogel, which then binds to lung endothelial cells lining the inside wall of the blood vessels where it rapidly delivers the drug.

The most potent of these, known as effector memory T cells, are activated by a group of proteins known as human leukocyte antigens (HLAs) on the surface of endothelial cells lining the donated organ's blood vessels.

Endothelial cells line the inside of all blood vessels and regulate inflammation, blood clotting following injury and blood pressure levels.

Endothelial cells lining blood vessels help keep what belongs in and what doesn't belong out.

Endothelial cells line the walls of the body's entire circulatory system, from the arterial walls of the heart to the smallest, most vulnerable capillaries — and their role in heart health is critical.

The corneal endothelial cells line the inner portion of the cornea.

Not exact matches

The Weill Cornell researchers» process was more direct: Four transcription factors prompted adult mouse endothelial cells, which line the inside of blood vessels, to turn into HSCs.

In experiments conducted on human lung endothelial cells and in mice, the researchers showed that NS1 caused permeability of the endothelium, which lines the walls of blood and lymph vessels.

In their latest study, Guarente and his colleagues decided to explore the role of sirtuins in endothelial cells, which line the inside of blood vessels.

In earlier studies involving animal models and human cancer cell lines, researchers found that breast cancer spreads when three specific cells are in direct contact: an endothelial cell (a type of cell that lines the blood vessels), a perivascular macrophage (a type of immune cell found near blood vessels), and a tumor cell that produces high levels of Mena, a protein that enhances a cancer cell's ability to spread.

In the current study, the researchers report that NOTCH1 acts like a sensor on the endothelial cells — the cells that line the valve and vessels — detecting blood flow outside of the cell and transmitting information to a network of genes inside the cell.

The researchers then differentiated the iPS cells into endothelial cells, which line the interior of the heart and blood vessels, before transplantation.

Using nanoparticles designed and screened for endothelial delivery of short strands of RNA called siRNA, the researchers were able to target RNAi to endothelial cells, which form the linings of most organs.

To determine whether endothelial cells — the cells that line the interior surface of blood vessels — directly influence breast cancer cell growth, they then created unique organotypic models of lung and bone marrow microvascular niches, in which endothelial cells formed blood vessel - like structures in culture as they would in the original organ.

In the chamber, tubes about the thickness of a human hair were lined with endothelial cells as in natural blood vessels.

Using a powerful imaging technique that allowed the scientists to track the presence and movement of parasites in living tissues, the researchers found that Toxoplasma infects the brain's endothelial cells, which line blood vessels, reproduces inside of them, and then moves on to invade the central nervous system.

«Endothelial cells make up the inner lining of blood vessels,» said William Durante, Ph.D., professor of medical pharmacology and physiology at the MU School of Medicine and lead author of the study.

The team used light and electron microscopy to show that lymphatic endothelial cells, which line the interior surface of the lymph node capsule, contain a number of channels that penetrate the whole thickness of the cells.

The study, published in Proceedings of the National Academy of Sciences, found that rod - shaped nanoparticles — or nanorods — as opposed to spherical nanoparticles, appear to adhere more effectively to the surface of endothelial cells that line the inside of blood vessels.

It both promotes angiogenesis within the tumor and increased the migration of endothelial cells, which form the inner lining of blood vessels.

«Up to now, the Tie2 receptor has only been known in endothelial cells that line the inside of blood vessels,» Milde said.

Instead of dispensing ink, Organovo's bioprinter uses two robotic tips to deposit globs of cells — in this case, endothelial cells that line blood - vessel walls, smooth muscle cells that regulate vessel dilation and contraction, and structural fibroblast cells.

Among other effects, immune cells disrupt the function of the endothelial layer, the lining of the blood vessels, counteracting «all the good things that the endothelial cells produce,» says physiologist Brett Mitchell of Texas A&M College of Medicine in College Station.

In an effort to overcome these limitations, a team at the Wyss Institute for Biologically Inspired Engineering led by its Founding Director, Donald Ingber, M.D., Ph.D., had previously engineered a microfluidic «Organ - on - a-Chip» (Organ Chip) culture device in which cells from a human intestinal cell line originally isolated from a tumor were cultured in one of two parallel running channels, separated by a porous matrix - coated membrane from human blood vessel - derived endothelial cells in the adjacent channel.

Malaria parasites grow in red blood cells and stick to the endothelial lining of blood vessels through a large family of parasite proteins called PfEMP1.

That's because experiments conducted on the International Space Station involving cells that line the inner surfaces of blood vessels (endothelial cells) show that microgravity accelerates cardiovascular disease and the biological aging of these cells.

To study this barrier and determine why a lack of blood flow causes it to leak, the researchers built a blood - vessel - on - a-chip model consisting of a channel lined with a layer of human endothelial cells surrounded by extracellular matrix within a microfluidic device, which allowed them to easily simulate and control the flow of blood through a vessel and evaluate the cells» responses.

The force of that blood flow helps keep the cells that line the blood vessels, called endothelial cells, healthy; when blood flow is disrupted, such as during surgical procedures or a stroke, the vessels start to leak, which can cause a host of inflammatory responses that lead to cell damage and disease.

The transplanted stem cells had differentiated into endothelial cells — which form the inner lining of a blood vessel, providing a barrier between blood and spinal cord tissue — and attached to capillaries in the spinal cord.

This environment includes endothelial cells (the cells lining the inside of blood vessels) and extracellular matrix (ECM) components, which provide structural support and signaling to surrounding cells.