Sentences with phrase «endothelial cells as»

Cell lineage tracing analysis helped researchers identify early endothelial cells as the cell of origin for the tumor subtype.

In addition, when these transcription factors lose their function, terminal differentiation into the vascular endothelium (completion of differentiation) is completely suppressed, and genes that are key to differentiation into vascular endothelial cells as well as transcription factors that maintain the undifferentiated state are adversely induced.

In the chamber, tubes about the thickness of a human hair were lined with endothelial cells as in natural blood vessels.

Not exact matches

Using their nuclei to exert force, they insert themselves between — as well as into — the cells (called endothelial cells) in the vessel walls.

Even as researchers test these findings in animals, Nabel's team is exploring an intriguing possibility: adding the Ebola virus membrane protein to a harmless virus that could then carry therapeutic genes specifically to endothelial cells.

«Overall, the most important finding is that we identified nanchangmycin as a potent inhibitor of Zika virus entry across all cell types tested, including endothelial and placental cells, which are relevant to how Zika may enter the fetus,» Cherry said.

And they incorporated into the gel a protein known as vascular endothelial growth factor (VEGF), which encourages the growth of blood vessels into the transplanted cells.

In a previous related study published in the Journal of Materials Science: Materials in Medicine, the same team of NTU scientists found that fish scale - derived collagen would induce human umbilical vein endothelial cells to express 2.5 times more of a specific type of collagen responsible for blood vessel formation, as compared to endothelial cells cultured on bovine collagen.

In another test, the researchers looked to see if chemical signals released from the endothelial cells would cause the media layer to relax and constrict, as they do in the human body.

In a collaborative effort between the Gladstone laboratories of Benoit Bruneau, PhD, Katherine Pollard, PhD, and Dr. Srivastava, the scientists used stem cell technology to make large amounts of endothelial cells from patients with CAVD, comparing them to healthy cells and mapping their genetic and epigenetic changes as they developed into valve cells.

To determine whether endothelial cells — the cells that line the interior surface of blood vessels — directly influence breast cancer cell growth, they then created unique organotypic models of lung and bone marrow microvascular niches, in which endothelial cells formed blood vessel - like structures in culture as they would in the original organ.

After a week, they saw endothelial cells in the brain that were infected, as well as evidence that the parasite was reproducing inside those cells.

They found that, while the grafts of undifferentiated iPS cells harbored large numbers of T cells of only a few specificities — indicating a robust immune response — those found in grafts of the two types of endothelial cells were more diverse, suggesting a more limited response associated with a phenomenon known as self - tolerance.

In the current study, the researchers showed that FGPs are present on the surface of the zebrafish brain and that these blood vessel - associated FGPs do not arise from the immune system, as had been previously thought, but from endothelial cells themselves.

Additionally, the newly described proliferating cells hold the promise of being able to regenerate a person's own endothelial cells, even in cases of FECD, as an alternative to transplanting corneas from a donor.

This process, known as necroptosis, enabled cancer cells to overcome an endothelial cell layer in the laboratory.

The study, published in Proceedings of the National Academy of Sciences, found that rod - shaped nanoparticles — or nanorods — as opposed to spherical nanoparticles, appear to adhere more effectively to the surface of endothelial cells that line the inside of blood vessels.

Researchers from Massachusetts Eye and Ear have, for the first time, identified rapidly proliferating cells (known as «neural crest - derived progenitor cells») in the corneal endothelium of specimens from normal corneas and from corneas with Fuchs» Endothelial Corneal Dystrophy (FECD), a condition in which the cells responsible for keeping the cornea clear die prematurely — often leading to blindness.

«ECPR and a factor in the blood called protein C act as a «brake» on blood coagulation and endothelial cell inflammation and also enhance the viability and integrity of blood vessels, but when the malaria parasites use PfEMP1 to bind EPCR, they may interfere with the normal function of EPCR, and thus the binding can be the catalyst for the violent reaction,» he explains.

The classical concepts of angiogenesis consider the endothelial cells within tumors as a rather passive cell population that merely reacts in response to growth factors released by the tumor cells.

They focused towards this end on the vascular growth factor Angiopoietin - 2, which is produced by endothelial cells and does not just control angiogenesis, but acts as a key responsiveness factor for endothelial cells.

The force of that blood flow helps keep the cells that line the blood vessels, called endothelial cells, healthy; when blood flow is disrupted, such as during surgical procedures or a stroke, the vessels start to leak, which can cause a host of inflammatory responses that lead to cell damage and disease.

Blood vessel endothelial cells act as «gatekeepers», the researchers write in their study published in the journal Scientific Reports.

As shown in Fig. 4B, suppressive ability of miR - 7 on both transmigration through endothelial cells and Matrigel was significantly attenuated by ectopic expression of KLF4, suggesting that miR - 7 suppresses invasive ability of CSCs by blocking KLF4 expression.

As expected, all of the endothelial cell lines showed diminished constitutive expression of E-selectin (data not shown) and VCAM - 1 (Fig. 4C) ⇓ in comparison with surface levels of ICAM - 1 (Fig. 4B) ⇓.

However, because IFN - γ is widely known to influence several endothelial properties, such as junctional integrity (21) and adhesion molecule expression (22), we elected to remove this cytokine from cultures immediately after the second session of cell selection.

Endothelial cells from heart, bladder, and colon provided notable exceptions as they continued to proliferate despite the transition to 37 °C.

The onset of tube structure formation could be visualized as early as 4 h after plating for most endothelial cell lines, with extensive networks becoming visible within 12 h after plating.

The endothelial cells in insulin - receptor knockout mice expressed more of the VCAM - 1 adhesion molecule that helps white blood cells grab onto the growing plaques, and blood vessels gathered four times as many white blood cells.

Such a depot or niche of endothelial stem - like cells would be analogous to the epithelial stem cells located in the anterior limbus that supply cells to the basal epithelium as transient amplifying cells, the cells that become committed to the state of terminal differentiation [11,15].

BALB / c corneal epithelial and endothelial cell cultures were established as described previously (23).

They served, therefore, as a comparison to human corneal endothelial cell division.

In the case of rabbit corneal endothelial cells, which are known to divide with regularity, AP - BrdU staining was always seen following a mechanical wound, as shown in Figure 3.

The extensive apoptosis of allogeneic corneal endothelial cells by CD8 − T cells from CD4 KO mice was perplexing, as this cell population should not contain CD4 + cells.

Evidence suggests that cells from this area migrate (perhaps as transient amplifying cells) to the endothelial periphery and, perhaps, to wounded areas of the corneal endothelium when needed.

Haematopoietic and endothelial cells arise from a common precursor, commonly referred to as the haemangioblast.

Angiopoietin - 2 functions as an autocrine protective factor in stressed endothelial cells.

Organ - on - a-chip technology may in part overcome this limitation, as exemplified by the «breathing» lung - on - a-chip that recapitulates the alveolar - capillary interface by co-culturing human alveolar epithelial cells and capillary endothelial cells on opposite sides of a flexible, porous, ECM - coated membrane.

Arap W and Pasqualini R Engineered embryonic endothelial progenitor cells as therapeutic Trojan horses.

The formation of new blood vessels from existing vessels, known as angiogenesis, depends on a sequential process that involves endothelial cells (EC) and pericytes1.

Modulating ECM assembly to alter tumor vascular barrier function Weilan Ye, Genentech Lymphangiogenesis and lymphatic endothelial barrier as targets in regenerative medicine Giorgia Jurisic, Novartis Wnt / beta - catenin signaling as a therapeutic target for blood - brain barrier repair in neurological disorders Dritan Agalliu, Columbia University Somatic mutations in hematopoietic cells contribute to cardiovascular disease: New mechanisms, new targets Kenneth Walsh, Boston University

Cardiovascular disease and renal disease for example are accelerated in patients with diabetes and AGEs have been shown to induce senescence in cell types related to these diseases, such as endothelial progenitor cells, vascular endothelial cells and renal cells.

The most potent of these, known as effector memory T cells, are activated by a group of proteins known as human leukocyte antigens (HLAs) on the surface of endothelial cells lining the donated organ's blood vessels.

â $ œWe have solved a major obstacle of using anti-miRNA as a therapeutic by being able to do a targeted delivery to only inflamed endothelial cells while all other tissues examined, including liver, lung, kidney, blood cells, spleen, etc showed no detectable side - effects, â $ Jo says.

The SPG cell layer, which is adjacent to the neurons of the brain, forms septate junctions, which function as a barrier to separate the humoral space and the brain, analogously to the mammalian tight junctions formed between endothelial cells.

Figure 6 shows the effects of 75 µM kahweol on endothelial cell migration, as determined by the «wound healing» assay, after 8 and 24 h of treatment.

As specific examples, our studies seek to define the molecular basis for platelet and endothelial cell responses and then extend these into patients to determine whether newly identified mechanisms can be targets for diagnosis, prevention and treatment of cardiovascular diseases.

They include its effects on endothelial cell «differentiation» to yield tubular - like structures, endothelial and tumor cell proliferation, apoptosis, and migration, as well as its effects on extracellular matrix remodelling enzyme activities of matrix metalloproteinase - 2 (MMP - 2) and urokinase - type plasminogen activator (uPA).

Human umbilical vein endothelial cells (HUVEC) were isolated by a modified collagenase treatment, as previously reported [43], and maintained as we described elsewhere [10].

These scientific goals guide our efforts at determining the role and mechanisms of the stromal cells, including the fibroblasts, endothelial and lymphatic elements, the matrix and the inflammatory / immune cells as critical targets for therapeutic intervention.