Sentences with phrase «endothelial cells plated»

In vitro, endothelial cells plated on Matrigel align themselves forming cords, already evident a few hours after plating.

In vitro, endothelial cells plated on Matrigel align themselves forming cords (Figure 5, C −, negative controls).

Endothelial cells plated onto two - chamber slides at a density of 2 × 105 cells / well in 10 % DMEM were incubated overnight.

Similar to cells growing at 33 °C, endothelial cells plated in the 37 °C environment also engaged in cell division during the first 24 h. However, during the next 72 h, the growth of most endothelial cells in 37 °C conditions dropped to 50 % of that of their counterparts growing at 33 °C (data not shown).

Not exact matches

Endothelial cells (passage 5) from each line were plated onto 96 - well plates at a density of 1000 cells / well in 10 % DMEM.

Matrigel - containing plates were allowed to incubate at 37 °C for 30 min and then seeded with endothelial cells at a density of 4 × 104 cells / well in 10 % DMEM.

The onset of tube structure formation could be visualized as early as 4 h after plating for most endothelial cell lines, with extensive networks becoming visible within 12 h after plating.

Briefly, single - cell suspensions of C57BL / 6 KO and wild - type lymphocytes, which had undergone a 96 hr in vitro boost with γ - irradiated (3,000 rad) BALB / c stimulator lymphocytes, were added to 96 - well microtiter plates (Corning Inc., Corning, NY) along with 2 × 104 51Cr - labeled BALB / c corneal epithelial or endothelial cells in a total volume of 200 μl / well.

For induction of differentiation to mature endothelial cells, EPCs were plated at a high cell density (8 × 104 cells / cm2) on tissue culture treated flasks.