Sentences with phrase «endothelial cells which»

«There are not many, if any, known systems which can be used to restore cardiovascular endothelial cells which are already damaged, and Vitamin D3 can do it,» Malinski said.

Not exact matches

The Weill Cornell researchers» process was more direct: Four transcription factors prompted adult mouse endothelial cells, which line the inside of blood vessels, to turn into HSCs.

AA147 directed the stem cells to develop primarily into endothelial cells, which are essential for blood vessel formation.

So Daniel Anderson at the Massachusetts Institute of Technology exposed human bone marrow stem cells to biodegradable nanoparticles carrying the human gene for vascular endothelial growth factor (VEGF), which attracts blood vessels to injury sites.

The protein, they found, preferentially binds to human endothelial cells, allowing the retrovirus, which would not normally infect human cells, to enter them.

Dismantling structural filaments within the cytoskeletons — the internal skeletons — of the endothelial cells creates the large holes, which can be several microns in diameter.

Prof. Alon says: «Our study shows that the endothelial cells, which were thought to be dynamic assistants in this process of crossing of blood vessel walls, are really more responders to the «physical work» invested by the white blood cell motors and nuclei in generating gaps and crossing through blood vessels.»

«Overall, the most important finding is that we identified nanchangmycin as a potent inhibitor of Zika virus entry across all cell types tested, including endothelial and placental cells, which are relevant to how Zika may enter the fetus,» Cherry said.

In experiments conducted on human lung endothelial cells and in mice, the researchers showed that NS1 caused permeability of the endothelium, which lines the walls of blood and lymph vessels.

And they incorporated into the gel a protein known as vascular endothelial growth factor (VEGF), which encourages the growth of blood vessels into the transplanted cells.

A critical discovery in this research was finding that the novel progenitor has a receptor protein on its cell surface called KDR, or vascular endothelial growth factor receptor 2, which until now, was thought to be restricted to endothelial cells that form vessels, the progenitors for endothelial cells and the progenitors blood cells.

In their latest study, Guarente and his colleagues decided to explore the role of sirtuins in endothelial cells, which line the inside of blood vessels.

To do that, they deleted the gene for SIRT1, which encodes the major mammalian sirtuin, in endothelial cells of mice.

Finally, the team added a layer of the patient's own endothelial cells, which promote smooth blood flow, on the inside of the vessel.

Moreover, a substantial increase in circulating endothelial progenitor cells, which repair damaged blood vessels, was noted after consuming bread made from the ancient grain Verna.

To make their molecular delivery vehicles, the researchers combined the polymersomes with a protein called LRP1, which is abundant in the endothelial cells that make up the blood — brain barrier wall.

The researchers then differentiated the iPS cells into endothelial cells, which line the interior of the heart and blood vessels, before transplantation.

A team from Cold Spring Harbor Laboratory in Long Island, N.Y., reports that it staved off full - blown metastasis in mice by preventing mini-tumors in the lungs from recruiting stem cells called endothelial progenitors, which assemble into blood vessels to nourish the malignancy.

Using nanoparticles designed and screened for endothelial delivery of short strands of RNA called siRNA, the researchers were able to target RNAi to endothelial cells, which form the linings of most organs.

To determine whether endothelial cells — the cells that line the interior surface of blood vessels — directly influence breast cancer cell growth, they then created unique organotypic models of lung and bone marrow microvascular niches, in which endothelial cells formed blood vessel - like structures in culture as they would in the original organ.

Using a powerful imaging technique that allowed the scientists to track the presence and movement of parasites in living tissues, the researchers found that Toxoplasma infects the brain's endothelial cells, which line blood vessels, reproduces inside of them, and then moves on to invade the central nervous system.

Researchers from the UNC School of Medicine have discovered that cells called fibroblasts, which normally give rise to scar tissue after a heart attack, can be turned into endothelial cells, which generate blood vessels to supply oxygen and nutrients to the injured regions of the heart, thus greatly reducing the damage done following heart attack.

A byproduct of HO - 1 is carbon monoxide, which promotes the survival of endothelial cells.

To explain their findings, the authors suggest that resveratrol's vasodilating effects via endothelial cells might enhance infiltration of inflammatory cells into the central nervous system, which in turn might play a key role in the pathogenesis of MS.

The team used light and electron microscopy to show that lymphatic endothelial cells, which line the interior surface of the lymph node capsule, contain a number of channels that penetrate the whole thickness of the cells.

The Max Planck researchers then showed that less necroptosis of endothelial cells and less metastasis occur in genetically modified animals in which Death Receptor 6 is disabled.

Researchers from Massachusetts Eye and Ear have, for the first time, identified rapidly proliferating cells (known as «neural crest - derived progenitor cells») in the corneal endothelium of specimens from normal corneas and from corneas with Fuchs» Endothelial Corneal Dystrophy (FECD), a condition in which the cells responsible for keeping the cornea clear die prematurely — often leading to blindness.

Malinski's team has developed unique methods and systems of measurements using nanosensors, which are about 1,000 times smaller in diameter than a human hair, to track the impacts of Vitamin D3 on single endothelial cells, a vital regulatory component of the cardiovascular system.

It both promotes angiogenesis within the tumor and increased the migration of endothelial cells, which form the inner lining of blood vessels.

The epithelium's maturation into a villus intestinal epithelium with long finger - like extensions was helped along by co-culturing human intestinal microvascular endothelial cells on the opposite side of the shared matrix - coated porous membrane in the «vascular» channel where they assembled a surrogate blood vessel with a hollow lumen through which feeding medium was flowed.

Co-culture of endothelial cells (green) and pericytes (red) results in spheroids which are used to study the interactions of the two cell types in 3D.

To verify that the process worked, the endothelial cells with the inversion - corrected genes were transplanted into F8 deficient mice (mice with hemophilia A) and the mice started producing the F8 clotting factor on their own, which essentially cured them of hemophilia A.

A specific lung - targeting molecule is attached to the carrier's surface and the steriod, dexamethasone, an anti-inflammatory, is loaded into the nanogel, which then binds to lung endothelial cells lining the inside wall of the blood vessels where it rapidly delivers the drug.

In an effort to overcome these limitations, a team at the Wyss Institute for Biologically Inspired Engineering led by its Founding Director, Donald Ingber, M.D., Ph.D., had previously engineered a microfluidic «Organ - on - a-Chip» (Organ Chip) culture device in which cells from a human intestinal cell line originally isolated from a tumor were cultured in one of two parallel running channels, separated by a porous matrix - coated membrane from human blood vessel - derived endothelial cells in the adjacent channel.

The scientists in Augustin's laboratory consequently pursued preclinical tumor therapy experiments, which were aimed at not just blocking angiogenesis, but to also suppress the production of tumor - promoting growth factors in endothelial cells.

They focused towards this end on the vascular growth factor Angiopoietin - 2, which is produced by endothelial cells and does not just control angiogenesis, but acts as a key responsiveness factor for endothelial cells.

To study this barrier and determine why a lack of blood flow causes it to leak, the researchers built a blood - vessel - on - a-chip model consisting of a channel lined with a layer of human endothelial cells surrounded by extracellular matrix within a microfluidic device, which allowed them to easily simulate and control the flow of blood through a vessel and evaluate the cells» responses.

The force of that blood flow helps keep the cells that line the blood vessels, called endothelial cells, healthy; when blood flow is disrupted, such as during surgical procedures or a stroke, the vessels start to leak, which can cause a host of inflammatory responses that lead to cell damage and disease.

The transplanted stem cells had differentiated into endothelial cells — which form the inner lining of a blood vessel, providing a barrier between blood and spinal cord tissue — and attached to capillaries in the spinal cord.

This environment includes endothelial cells (the cells lining the inside of blood vessels) and extracellular matrix (ECM) components, which provide structural support and signaling to surrounding cells.

In this tissue system, we can culture patient - derived megakaryocytes — the bone marrow cells that make platelets — and also endothelial cells, which are found in bone marrow and promote platelet production, to design patient - specific drug administration regimes.»

The vessels are lined with endothelial cells, which secrete chemical signals that help stem cell survive.

This experiment uses quantitative PCR to detect the expression level of POSTN in CD34 + / CD31 − pulmonary fibroblasts, CD31 + endothelial cells, and CD45 + immune cells isolated from lungs of mice with macrometastases, which is a replication of the experiment reported in Figure 2H.

At that key point, Takebe added two more types of cell known to help to recreate organ - like function in animals: endothelial cells, which line blood vessels, taken from an umbilical cord; and mesenchymal cells, which can differentiate into bone, cartilage or fat, taken from bone marrow.

Thus, our selection strategies, which were based on targeting endothelial populations that expressed both of these inducible glycoproteins, suggest that the endothelial cell lines generated in this study are most likely from this microcirculatory region.

The study focuses on endothelial cells, the cells lining the inside of blood vessels in which atherosclerotic plaques develop.

The present demonstration of T cell - mediated apoptosis of allogeneic corneal cells from CD4 KO mice is consistent with previous findings, which noted the presence of apoptotic keratocytes and corneal endothelial cells in rejected corneal allografts in humans and rats respectively (5, 32).

The scientists began with a line of mice in which the gene for the insulin receptor has been knocked out in endothelial cells only.

In the case of rabbit corneal endothelial cells, which are known to divide with regularity, AP - BrdU staining was always seen following a mechanical wound, as shown in Figure 3.

Briefly, single - cell suspensions of C57BL / 6 KO and wild - type lymphocytes, which had undergone a 96 hr in vitro boost with γ - irradiated (3,000 rad) BALB / c stimulator lymphocytes, were added to 96 - well microtiter plates (Corning Inc., Corning, NY) along with 2 × 104 51Cr - labeled BALB / c corneal epithelial or endothelial cells in a total volume of 200 μl / well.