Next they were covered on
the endothelial side with a 1:10 dilution of anti-mouse-Ig secondary antibody coupled to alkaline phosphatase.
The human corneas had a 10 mM BrdU solution of Optisol placed onto
the endothelial side of dissected corneal - scleral buttons prior to assay.
The fixed tissues were covered on
the endothelial side with a 1:1000 dilution of anti-BrdU primary antibody.
Cornea buttons were mounted on an artificial anterior chamber,
endothelial side up.
The BrdU reagent (10 mM) was diluted 1:10 in Optisol and 120 μl was placed on a corneal - scleral button on
the endothelial side only.
Not exact matches
The epithelium's maturation into a villus intestinal epithelium with long finger - like extensions was helped along by co-culturing human intestinal microvascular
endothelial cells on the opposite
side of the shared matrix - coated porous membrane in the «vascular» channel where they assembled a surrogate blood vessel with a hollow lumen through which feeding medium was flowed.
The
endothelial cells have a receptor for transferrin that acts like a gatekeeper: When transferrin binds to a receptor on the blood
side of the barrier, the
endothelial cell transports it (and its iron cargo) to the other
side and spits it out into the brain.
Next,
endothelial primary cells (EPCs) were grown on one
side of the silk scaffold and megakaryocytes were seeded on the other
side.
Organ - on - a-chip technology may in part overcome this limitation, as exemplified by the «breathing» lung - on - a-chip that recapitulates the alveolar - capillary interface by co-culturing human alveolar epithelial cells and capillary
endothelial cells on opposite
sides of a flexible, porous, ECM - coated membrane.
July 13, 2016 Simple procedure could improve treatment for common eye disease: Rejuvenating the cornea could reduce the need for cornea transplants A new, minimally invasive procedure appears to be effective for many patients with Fuchs
endothelial dystrophy (FED), a common eye disease, without the potential
side effects and cost of the current standard of care, a cornea transplant.
â $ œWe have solved a major obstacle of using anti-miRNA as a therapeutic by being able to do a targeted delivery to only inflamed
endothelial cells while all other tissues examined, including liver, lung, kidney, blood cells, spleen, etc showed no detectable
side - effects, â $ Jo says.
The iPS cells are cultured on the opposite
side of the membrane in the other channel that represents the glomerulus» urinary compartment, where they are induced to form a layer of mature podocytes that extend long cellular processes through the pores in the membrane and contact the underlying
endothelial cells.
FUN FACT: If you laid your
endothelial cells
side by
side in a straight line, they would wrap about the earth FOUR TIMES!
I know the bad
side of fat for
endothelial function, but what about on telomeres?