Sentences with phrase «endothelial tissue from»

The endothelial tissue from these samples was dissected and samples were analyzed for mRNA transcription of p53, transactivating p63 (TAp63), delta N p63 (ΔNp63), transactivating p73 (TAp73), and delta N p73 (ΔNp73) via the reverse transcriptase - polymerase chain reaction (RT - PCR).

Not exact matches

When zebrafish with the green fluorescing endothelial gene matured, the researchers observed green FGPs on the surface of the fish's brains — confirming that these cells arose from endothelial tissue.

Researchers from the UNC School of Medicine have discovered that cells called fibroblasts, which normally give rise to scar tissue after a heart attack, can be turned into endothelial cells, which generate blood vessels to supply oxygen and nutrients to the injured regions of the heart, thus greatly reducing the damage done following heart attack.

To overcome these limitations, we used two - color flow cytometry to identify and select microvascular endothelial cells from primary cultures obtained from different organs of mice whose tissues harbor a temperature - sensitive SV40 large T antigen (H - 2Kb - tsA58 mice; ImmortoMice).

One of the major impediments to obtaining a large number of endothelial cells from different tissues has been the inability to purify and propagate these cells in culture.

Tissue - specific microvascular endothelial cell lines from H - 2K (b)- tsA58 mice for studies of angiogenesis and metastasis

In B, endothelial cells from different tissues were seeded at a density of 1 × 105 cells / chamber in two - chamber slides and incubated with 10 mg / ml DiI - Ac - LDL in 10 % DMEM for 4 h. Cells were washed with label - free medium and fixed in 4 % paraformaldehyde.

Figure 2 shows sets of results for four areas of human corneal endothelial tissue; central endothelium (defined by endothelial cells within a 4 mm trephined boundary), central - intermediate endothelium (defined by endothelial cells within an 8 mm trephined boundary), intermediate - peripheral endothelium (defined by endothelial cells between the 4 mm trephine edge and 12 mm from the center of the corneal endothelium), and the peripheral endothelium (defined by the 8 mm trephined edge and 12 mm from the center of the corneal endothelium).

For each assay, 200 μl of sample (taken from the Optisol solution bathing the human and rabbit corneal endothelial tissues) was added to microplates coated with TGF - β receptor.

We will combine methods from the fields of immunology, developmental biology and angiogenesis to understand in vivo the development and lineage - specific function (s) of resident macrophages, thereby opening new venues of research into the interaction between macrophages and endothelial cells in response to tissue damage.

Isolation of Endothelial Cells and Vascular Smooth Muscle Cells from Internal Mammary Artery Tissue.

They used a method to identify genes that are co-expressed with already known endothelial - enriched genes and analysed RNA sequencing data from 124 unprocessed tissue samples from 32 different organs.

Endothelial progenitor cells (EPCs) were isolated from these vascular tissues following standard collagenase digestion procedure.

The endothelial tissues within these separate sections were carefully stripped away from their underlying stromas.