Sentences with phrase «engineer zinc finger nucleases»

Eventually, the team of Dow and Sangamo scientists showed that it could engineer zinc finger nucleases that gave a two - for - one deal.

Dow Agrosciences wanted to see if engineered zinc finger nucleases could speed up genetic engineering.

Not exact matches

By using engineered zinc - finger nucleases (ZFNs) designed to target an integrated reporter and two endogenous rat genes, Immunoglobulin M (IgM) and Rab38, we demonstrate that a single injection of DNA or messenger RNA encoding ZFNs into the one - cell rat embryo leads to a high frequency of animals carrying 25 to 100 % disruption at the target locus.

Talk of curing AIDS made front - page news last year, in part due to an astonishing new gene - editing technology: lab - engineered proteins called zinc finger nucleases.

Here we describe engineering a pair of zinc finger nucleases that, when introduced into human T cells, efficiently disrupt cxcr4 by cleavage and error - prone non-homologous DNA end - joining.

Previously we engineered zinc - finger nucleases (ZFNs) to specifically disrupt the CCR5 gene in primary human T cells, the predominant cell type infected and killed by HIV.

Working with proteins called zinc finger nucleases and TALENs, Zhang attempted to edit the genomes of mammalian cells with a view to engineering them.

With the genome engineering revolution came epigenome - engineering tools - zinc finger nucleases and TALENs fused to epigenetic modifiers enabled epigenetic modifications at a user - specified locus.