Targeted drugs made from
engineered immune proteins — called monoclonal antibodies — have revolutionised treatment for several types of cancer in recent years.
Not exact matches
The team genetically
engineered immune cells so that a calcium gate - controlling
protein became light sensitive.
This is an illustration of how the
engineered protein facilitates destruction of latently HIV - infected
immune cells.
They then injected the
protein into elderly mice with normal
immune systems, and found this reproduced the beneficial effects of cord plasma on both memory performance and LTP in the hippocampus whereas mice
engineered to lack TIMP2 showed reduced LTP.
But rather than delivering the entire gene for the clotting - factor
proteins to cells, as most gene therapies do, the researchers used the viruses to
engineer immune - regulating B cells to express a fragment of the clotting factor fused to an
immune molecule called an immunoglobulin.
Now a team of
engineers at MIT has developed a new way to deliver such vaccines directly to the lymph nodes, where huge populations of
immune cells reside: These vaccines hitch a ride to the lymph nodes by latching on to the
protein albumin, found in the bloodstream.
Using a combination of human or specially
engineered mouse cells in vitro and in vivo animal models, study senior investigator Judy Lieberman, MD, PhD; study lead investigator Farokh Dotiwala, PhD, with a team lead by the Brazilian parasitologist Ricardo Gazzinelli, DSc, DVM, found that when an
immune killer cell, such as a T - cell or natural killer (NK) cell, encounters a cell infected with any of three intracellular parasites (Trypanosoma cruzi, Toxoplasma gondii or Leishmania major), it releases three
proteins that together kill both the parasite and the infected cell:
And, in fact, these doctors and researchers are finding incredible success with this strategy; for example, PD - 1 inhibitors remove this «cloak» that cancers use to hide from the
immune system, and CAR - T cell therapies use specially
engineered T - cells to seek cancer - specific
proteins and destroy the cancer cells to which they are attached.
Two types of vaccines were used for the study: one constructed with genetically
engineered DNA molecules that teach
immune system cells to recognize premalignant cells expressing HPV16 E7
proteins, and one that is a non-infectious,
engineered virus that targets and kills precancerous cells marked by HPV16 and HPV18 E6 and E7
proteins.
The technique could make drug - producing bacteria
immune to viruses, prevent laboratory
engineered organisms from genetically contaminating wildlife and enable scientists to construct
proteins that do not exist in nature.
By
engineering red blood cells to have «sticky»
proteins on their surface, a team of researchers has given the cells the ability to carry anything from drugs to treat
immune disorders or cancer to radioactive molecules used in imaging of blood vessels.
A central question has been answered regarding a
protein that plays an essential role in the bacterial
immune system and is fast becoming a valuable tool for genetic
engineering.
It contains an
engineered protein that combines a
protein fragment from the malaria parasite, Plasmodium falciparum, and a
protein from the Hepatitis B virus that helps trigger a strong
immune response.
The most advanced to date — dubbed RTS, S — tries to teach the
immune system to defeat the parasite with a genetically
engineered version of a
protein from Plasmodium falciparum, the strain that causes the most serious disease in humans.
To get to the bottom of this question, researchers in the Perelman School of Medicine at the University of Pennsylvania
engineered mice in which the damage caused by a mutant human TDP - 43
protein could be reversed by one type of brain
immune cell.
It is in this final tank that the
engineered cells are stimulated to secrete the
protein product — the monoclonal antibody itself, a
protein derived from the mammalian
immune system that can bind to a very specific target in the body, such as a tumor cell.
The awards span the broad mission of the NIH and include groundbreaking research, such as
engineering immune cells producing drugs at the site of diseased tissue; developing a sensor to rapidly detect antibiotic resistance of a bacterial infection; understanding how certain parasites evade host detection by continually changing their surface
proteins; and developing implants that run off the electricity generated from the motion of a beating the heart.
By
engineering an artificial
protein that targets the product of such potentially cancer - causing gene fusions, Hur hopes to trigger
immune defense mechanisms that kill rogue cells harboring the fusions.
The approach developed by the MGH team starts with the
engineered protein, which in this case fuses an antibody fragment targeting a
protein called mesothelin — expressed on the surface of such tumors as mesothelioma, ovarian cancer and pancreatic cancer — to a
protein from the tuberculosis bacteria that stimulates the activity of dendritic and other
immune cells.
Unmatched performance — high transfection efficiency & cell viability in primary cells Fully scalable — from R&D to the clinic without reoptimization Safe — non-viral cell
engineering in closed, sterile, computer - controlled environment Cell loading flexibility — mRNA, gRNA, siRNA, DNA,
proteins, cell lysates, and small molecules into autologous or allogenic
immune, stem / progenitor, or somatic cells Regulatory ease — Master File designation with the CBER Division of the U.S. FDA, cleared by NIH's RAC and Health Canada, cGMP - compliant, and CE - marked
To that end, she genetically
engineered mice to lack a
protein that transports cholesterol out of cells in order to analyze experimentally what happens when
immune cells remain abnormally packed with cholesterol.
As noted above, existing cancer vaccines that use dendritic cells require extracting cells from a patient's blood, treating them with an
engineered protein or nucleic acid that combines tumor antigens with
immune - stimulating molecules, and returning the activated dendritic cells to the patient.
The process uses an
engineering technique called reserve genetics and allows scientists to remove a
protein from both flu viruses, weakening them considerably, but still promoting an
immune system response in the dogs that won't make them sick.