Sentences with phrase «engineered nucleases»

According to Director Jin - Soo Kim, «We used CRISPR RGENs [RNA - guided engineered nucleases] to repair two recurrent, large chromosomal inversions responsible for almost half of all severe hemophilia A cases.»

Just this week, for example, a team led by Feng Zhang of the Broad Institute of Harvard and MIT, one of the pioneers of the method, published a paper in Science on engineering the nuclease part of CRISPR so that it more accurately cuts the intended DNA target.

Not exact matches

By using engineered zinc - finger nucleases (ZFNs) designed to target an integrated reporter and two endogenous rat genes, Immunoglobulin M (IgM) and Rab38, we demonstrate that a single injection of DNA or messenger RNA encoding ZFNs into the one - cell rat embryo leads to a high frequency of animals carrying 25 to 100 % disruption at the target locus.

Talk of curing AIDS made front - page news last year, in part due to an astonishing new gene - editing technology: lab - engineered proteins called zinc finger nucleases.

Here we describe engineering a pair of zinc finger nucleases that, when introduced into human T cells, efficiently disrupt cxcr4 by cleavage and error - prone non-homologous DNA end - joining.

Previously we engineered zinc - finger nucleases (ZFNs) to specifically disrupt the CCR5 gene in primary human T cells, the predominant cell type infected and killed by HIV.

Working with proteins called zinc finger nucleases and TALENs, Zhang attempted to edit the genomes of mammalian cells with a view to engineering them.

Genetic Engineering and Model Validation: engineering and generation of customised genetically modified mouse models, generation of genetically modified ES cells, genotyping, analysis of gene expression, model validation by molecular analysis, CRISPR / Cas9 nucleases technology

With the genome engineering revolution came epigenome - engineering tools - zinc finger nucleases and TALENs fused to epigenetic modifiers enabled epigenetic modifications at a user - specified locus.

This protocol describes a system for efficient genome editing in hPSCs using engineered transcription activator - like effector nucleases (TALENs) or clustered regularly interspaced short palindromic repeat (CRISPR) technology.

We have recently developed the use of site - specific nucleases to genetically engineer human pluripotent stem cells, which can be maintained indefinitely and differentiated into any cell type of interest.

Dow Agrosciences wanted to see if engineered zinc finger nucleases could speed up genetic engineering.

Eventually, the team of Dow and Sangamo scientists showed that it could engineer zinc finger nucleases that gave a two - for - one deal.