Sentences with phrase «enhancers in embryonic stem cells»
We'll start with ChIP - seq data from the ENCODE project, find enhancers in embryonic stem cells, and conclude with a targeted CRISPR screen that can disrupt these enhancers.
http://blog.addgene.org/ Not exact matches
Furthermore, by making use of embryonic stem cells and in vitro differentiation, SIF - seq can be used to assess enhancer activity in a wide variety of disease - relevant cell types.»
The ability of SIF - seq to use reporter assays in mouse embryonic stem cells to identify human embryonic stem cell enhancers that are not present in the mouse genome opens the door to intriguing research possibilities as Dickel explains.
«We've shown that SIF - seq can be used to identify enhancers active in cardiomyocytes, neural progenitor cells, and embryonic stem cells, and we think that it has the potential to be expanded for use in a much wider variety of cell types,» Dickel says.
The findings are reported in the article «A Sox2 distal enhancer cluster regulates embryonic stem cell differentiation potential» published online December 15 in Genes & Development.
With the new SIF - seq technique, mouse embryonic stem cells can be used to identify human embryonic stem cell enhancers even when the human enhancers are not present in the mouse genome.