Not exact matches
In the new study, the researchers
sequenced the
entire genome of mice that had undergone CRISPR gene editing in the team's previous study and looked for all mutations, including those that only altered a single
nucleotide.
The third is a method implemented by Reich for reading the genetic codes of 1.2 million carefully chosen variable parts of DNA (known as single
nucleotide polymorphisms) rather than having to
sequence entire genomes.
The human genome contains about 3 billion base pairs, but only about 2 percent of these base pairs represent protein - coding genes, meaning that whole - exome
sequencing measures the genetic alterations focused on a small but very important fraction of the genome (as opposed to techniques of whole genome
sequencing, which measures every
nucleotide across the
entire genome, regardless of whether these genes are expressed or silent).
To reveal the structural genetic code, researchers examine chromatin from its
sequence of
nucleotides to the organisation of an
entire genome.
Genomic
sequencing of the isolated virus revealed that it is closely related to Tembusu virus (a mosquito - borne Ntaya group flavivirus), with 87 — 91 %
nucleotide identity of the partial E (envelope) proteins to that of Tembusu virus and 72 % of the
entire genome coding
sequence with Bagaza virus, the most closely related flavivirus with an entirely
sequenced genome.
Molecular evolutionary analysis of the
entire coding
sequence in the eight representative mammalian species: Monotremes (platypus), Marsupials (opossum) and Eutherians (mouse, rat, dog, rhesus monkey, chimpanzee and human), revealed no excessive non-synonymous
nucleotide changes in comparison with synonymous changes.