Sentences with word «enucleated»

A team in the laboratory of Atsuo Ogura at the National Institute of Infectious Diseases in Shinjuku, Tokyo, cloned 12 mice by removing nuclei from testis cells and inserting them into enucleated egg cells.

King, T. J. and Briggs, R. Transplantation of Living Nuclei of Late Gastrulae into Enucleated Eggs of Rana pipiens.

Wakayama, T., Perry, A. C. F., Zuccotti, M., Johnson, K. R., and Yanagimachi, R. Full - Term Development of Mice from Enucleated Oocytes Injected with Cumulus Cell Nuclei.

Additionally, one study (Esson et al) has theorized that uveitis is not present in GRPU, based on histopathologic evaluation of eyes enucleated due to glaucoma.4 However, other authors believe that uveitis is indeed present, and that the eyes from the Esson study showed little evidence of uveitis because the eyes had been treated with anti-inflammatory medication prior to their removal.

If the eye was enucleated due to a severe tear or rupture, the eye may not be removed in one piece.

Many entities that are not embryos (teratomas, parthenotes, complete hydatidiform moles, and even enucleated oocytes) resemble embryos to varying degrees and yet can be reliably distinguished from embryos by careful comparison.

The resulting cells are themselves fused with other enucleated egg cells.

Other researchers have previously cloned animals, including mammals, by transferring nuclei from embryonic cells into such enucleated eggs.

Oocytes with a first polar body were enucleated manually in the presence of 7.5 µg / ml of cytochalasin B.

Differentiation of this heterozygous iPSC line to erythrocytes used an improved feeder - free and xeno - free three - step culture strategy, generating enucleated hemoglobin expressing cells to a similar level to that observed for the parental TNC1 iPSC line (see figure).

They injected intact nuclei from the last living ibex into more than 400 enucleated eggs of the domestic goat.

To further complicate matters, eyes enucleated from dogs with GRPU often demonstrate tiny iridociliary cysts microscopically but cysts are often not detectable clinically in these same eyes prior to enucleation.

OAR proponents claim that when the altered donor - cell nucleus with its activated nanog gene is transferred to the enucleated oocyte (egg cell), the presence of nanog will immediately convert the enucleated egg cell to a pluripotent cell, without ever forming a zygote.

Then, the DNA would be removed from an oocyte (an egg cell) and this enucleated oocyte fused to the altered adult cell» creating a new cell that is neither an oocyte nor an adult cell but a hybrid exhibiting the properties programmed into it by the alterations made to the adult - cell nucleus.

Indeed, the ability to clone animals, such as Dolly the sheep, by fusion of an adult cell to an enucleated oocyte demonstrates that the epigenetic programming responsible for maintaining an adult cell in a stable state can be erased by factors present in the cytoplasm of the oocyte.

The cell produced following fusion of such an altered adult nucleus with an enucleated oocyte would be capable of generating embryonic stem cells» yet fundamentally incapable of orchestrating an overall embryonic pattern of development.

The enucleated cell is akin to the state of being braindead, in that it lacks a full critical complexity.

If so, they might be able to transplant a white blood cell nucleus into an egg from a frog or other animal that has been enucleated, or stripped of its own nucleus.

Rather than transferring just the nucleus, the ABS workers fuse a whole donor cell with an enucleated (nucleus removed) egg cell, a process that is helped along with a jolt of electricity.

The only alternative for generating patient - specific cell lines is a process called therapeutic cloning, a technique that involves transplanting the nucleus from an adult cell into an enucleated egg.

The only way to do this now is by nuclear transfer to an enucleated egg cell («therapeutic cloning»).

But the next step — transferring the nucleus — was different; rather than fusing the donor cell with the enucleated egg cell, the team removed the donor nucleus with a very fine needle and injected it into the donor egg.

In 2003, Spanish scientists injected intact nuclei from the last living Pyrenean ibex, a wild goat endemic to the French mountain range, into more than 400 enucleated eggs of the domestic goat.

He tested various methods to quickly and precisely remove the nuclear materials from the egg cell and promote the fusion of the nucleus - donor cell and enucleated egg.

You take that cell and fuse it to the enucleated egg, activate it — which starts it growing — and transfer it to a surrogate mother.

Until recently, scientists believed the way to do this would be through therapeutic cloning, a controversial technique — still unproven for humans — that involves putting the nucleus from a body cell into an enucleated egg.

Collins writes, «I would argue that the immediate product of a skin cell and an enucleated egg cell fall short of the moral status of the union of sperm and egg» (pg.

Wilmut and his colleagues transplanted a nucleus from a mammary gland cell of a Finn Dorsett sheep into the enucleated egg of a Scottish blackface ewe.

However, one line showed a significant increase in the proportion of the carried - over mtDNA haplotype compared with that in the enucleated egg.

During the 1950s and 1960s, researchers working with frogs developed techniques to extract the nucleus of a differentiated adult cell and stick it into an enucleated, unfertilized egg.

In 1996 researchers in Scotland used SCNT to transplant the nucleus of an adult sheep cell into an enucleated sheep egg, producing Dolly, the first cloned mammal.

In essence, these studies suggested that a newly fertilized, enucleated egg has the ability to «reprogram» a much older donor nucleus, making the resultant cell behave as though it were at a very early embryonic state.

The next steps in cloning would be to 1) insert a donor nucleus of a new bird into the enucleated egg cell, 2) stimulate embryonic growth, and 3) implant the embryo into a mother bird or egg.

In the early 1950s, Robert Briggs and Thomas King repeated Spemann's experiments using a species of leopard frog, Rana pipiens, first with a nucleus from young embryos (Briggs and King, 1952) then from older embryos (King and Briggs, 1954); both the younger and older implanted nuclei could still be reprogrammed by the enucleated host cell.

SCNT is a technique wherein the nucleus from a somatic cell (an adult cell that is not a sperm or egg, i.e. not the gametes) is implanted into an enucleated egg cell which can then be implanted into, and develop in, a surrogate mother, and potentially become an adult organism.

In 1938, Hans Spemann showed that a nucleus from a fertilized salamander egg that had already undergone cell division several times could be implanted into a cell from a newly fertilized salamander egg that is enucleated (has had its nucleus removed) and create an entire adult salamander (Spemann, 1938).

An adult cell from the mammary gland of a Finn - Dorset ewe acted as the nuclear donor; it was fused with an enucleated egg from a Scottish Blackface ewe, which acted as the cytoplasmic (or egg) donor.

The image here shows the process of the Honolulu technique when the nucleus is injected into an enucleated oocyte.

The procedure transplants nuclear DNA from an egg of a woman with mitochondrial disease into an enucleated egg cell from a woman with healthy mitochondria.

Eye was enucleated, doing great.»

Brachycephalic breeds, those with flattened faces and prominent eyes, tend to be predisposed to eye injuries and often it is these breeds that end up with one eye enucleated.