Confocal images were taken at days 1, 2, 3, 4, 5, 6, 8, and 10, on another set of roots allowing the measurement and counting of the cells along single
epidermal cell files.
Once identified within the meristem, the hair
epidermal cell file was followed into the elongation zone.
Not exact matches
To confirm, evaluate, and quantify the extent of such exponential behavior in individual plant roots, we analyzed root
epidermal (trichoblast, to be able to recognize root hair) and cortex
cell files of wild ‐ type Col ‐ 0 ecotype, from day 1 to 10 postgermination (Materials and Methods, Dataset EV1).
Epidermal and cortical
cells were measured individually in each plant along a single
file and each analyzed time point using ImageJ software (http://rsb.info.nih.gov/ij/).
Morphologically, the DZ in the wild type (WT) is marked by the appearance of
epidermal root hairs on the external surface of outer
cell files (trichoblast) and the existence of fully differentiated xylem for internal
cell files (Dolan et al, 1993; Ishikawa & Evans, 1995; Beemster et al, 2003; Verbelen et al, 2006; Zhang et al, 2010; Mähönen et al, 2014).