Interestingly, long - term — cultured human
epithelial cancer cells in clonal cultures also form holoclones, meroclones, and paraclones, and tumor cell holoclones have been hypothesized to harbor stem - like cells or cancer stem cells.
The observations made herein are unlikely restricted to only PC3 cells as Du145 holoclones can also be serially passaged and sustain long - term propagation (31) and many other
epithelial cancer cells form holoclones that also express CSC markers such as CD44 (30).
Similar to primary keratinocytes, long - term — cultured squamous cell carcinoma (30) and many other
epithelial cancer cells (30, 31) can also form different types of clones in culture.
We use multiple mouse models combined with in vitro approaches to address the pleiotropic functions of these cytokines which affect both
the epithelial cancer cells and the inflammatory infiltrate within tumors.
Not exact matches
The scientists identified several, including the investigational
cancer drug BEZ235, which blocked a key metabolic pathway in flu - infected human lung
epithelial cells.
Lead author Moustafa Abdalla writes: «Almost all genomic studies of breast
cancer have focused on well - established tumours because it is technically challenging to study the earliest mutational events occurring in human breast
epithelial cells.»
Dr. Done explains: «Most breast
cancer starts in the
epithelial cells lining the mammary ducts.
Indomethacin killed both drug - resistant and drug - sensitive
epithelial ovarian
cancer cells.
Interestingly, cisplatin - resistant
epithelial ovarian
cancer cells were most sensitive to indomethacin.
In one experiment, the researchers created arrays of mammary
epithelial cells and asked how adding one or more
cells expressing low levels of the
cancer gene RasG12V affected the
cells around them.
Almost 80 percent of
cancers have
epithelial cell origin.
«We showed that PLK1 drives migration of normal prostate
epithelial and prostate
cancer cells through an entirely different process.»
Recently published in the journal eLife and featured in a poster session at this year's American Association for
Cancer Research (AACR) annual meeting, preclinical experiments show for the first time that PLK1 promotes the migration of prostate cancer cells by setting in motion a process that induces what is known as the epithelial - to - mesenchymal transition
Cancer Research (AACR) annual meeting, preclinical experiments show for the first time that PLK1 promotes the migration of prostate
cancer cells by setting in motion a process that induces what is known as the epithelial - to - mesenchymal transition
cancer cells by setting in motion a process that induces what is known as the
epithelial - to - mesenchymal transition (EMT).
The new research finds that the stiffness of the extracellular matrix and the availability of certain ligands (molecules that bind to
cell membranes) can together determine which genes are actually called on — and whether normal
epithelial cells begin to exhibit the behaviors characteristic of highly malignant
cancer cells.
By manipulating it in vitro, a team of researchers led by Prof. David Mooney at Harvard SEAS have identified a possible mechanism by which normal
cells turn malignant in mammary
epithelial tissues, the tissues frequently involved in breast
cancer.
But by hierarchical clustering of observed alterations in endocytosis, the researchers identified two distinct
cancer phenotype clusters, one marked by mutations in the oncogene KRAS in mesenchymal
cells and the other by changes in
epithelial cells.
The images show how a growth factor caused
cells to change forms and regroup from tight packs of
epithelial cells to more mobile, loose arrays of mesenchymal
cells — information useful for studying
cancer and wound healing.
The
cell changes, known as an
epithelial to mesenchymal transition, or EMT, are normal and helpful during wound healing, but problematic when
cancer cells spread from the primary tumor site to other sites in the body.
«It is striking that these changes, found in many human
cancers, can be induced in normal mammary
epithelial cells simply by varying the stiffness or composition of the matrix surrounding them.»
Researchers from the University of Michigan and the Mayo Clinic studied rat IEC - 6 intestinal
epithelial cells, chosen because they maintain a stable diploid genomic structure (two sets of chromosomes), lack the cellular characteristics of
cancer cells, and replicate normally.
HER2 - positive breast
cancer, which is associated with poor prognosis, has been traced to an excess of signaling through receptors, or docking ports, called HER2 found on the surface of certain mammary
epithelial cells.
The researchers found that this loss of identity allows the relatively well - behaved
epithelial cells to transition to bad - acting mesynchemal
cells, which are more likely to move throughout the body — the hallmark of metastatic
cancer, which is the primary cause of
cancer recurrence and patient death.
The goal of the first experiment was to see whether lncRNAs are differentially expressed in prostate
cancer by measuring total RNA from prostate
cancer cell lines and normal
epithelial prostatic
cells using NCode human ncRNA array and SurePrint G3 human lncRNA microarrays.
Researchers have built a model to investigate the metastasis of
cancer by examining the metabolism of breast
epithelial cells and look at the role of signaling.
In addition, the combination of defective
epithelial barrier and abnormal
epithelial cell extrusion observed in this mouse model may be further exploited to study the role of Alix in
cancer.
In search of early markers of lung
cancer, pulmonary and critical care physicians Avrum Spira and Jerome Brody of the Boston Medical Center sampled bronchial tube
epithelial cells from 85 people.
In a study of 51 patients, researchers used a state - of - the art microfluidic device to capture circulating pancreas
epithelial cells in 33 percent of patients with early pancreatic lesions and no clinical diagnosis of
cancer.
Additionally, overexpression of POSTN in human mammary
epithelial and breast
cancer cells resulted in enhanced tumor growth and metastasis (Wang et al., 2013), which is similar to a colon
cancer cell model where overexpression of POSTN resulted in an increase in the number and size of liver metastases (Bao et al., 2004).
Researchers captured circulating
epithelial cells in 73 percent of patients with
cancer.
Human breast
cancer cells generated by oncogenic transformation of primary mammary
epithelial cells.
Once the bacterial
cell signaling protein cagA reaches the host cytosol, it is capable of altering subsequent generations of progenitor
cells, leading to the development of
cancer through changes in mitotic activity, apoptosis, cellular assembly, and signaling.26 Although it should be noted that while the presence of the cagA protein doubles the risk of gastric
cancer, cagA - negative strands also increase the risk of distal gastric
cancer.27 Additional H pylori virulence factors include babA2, which encodes bacterial adhesion with gastric
epithelial cells, and vacuolating cytotoxin A, which is encoded by the gene vacA.28, 29 H pylori strains carrying some combination of the babA2, cagA, and vacA genes were associated with the highest risk of developing intestinal metaplasia.
When normal
cells are transformed into
cancer cells, this
epithelial tissue can take on the characteristics of embryonic tissue, known as mesenchymal tissue, which is comprised of unspecialized
cells that will develop, as the embryo matures, into more specialized tissues.
The Joslin team found that intestinal tumors grow just as quickly in a mouse
cancer model whose «
epithelial»
cells lining the inside of the intestine have been genetically modified so that insulin can't activate the
cells.
Interestingly, suppression of Dicer activities is reported to result in promotion of
epithelial - mesenchymal transistion and metastatic transformation of
cancer cells.
Contribution of Xanthine Oxidoreductase to Mammary
Epithelial and Breast
Cancer Cell Differentiation In Part Modulates Inhibitor of Differentiation - 1.
However, up to five-fold higher levels of activated matriptase were detected in the conditioned media from the
cancer cells grown in the absence of serum and S1P, when compared to non-transformed mammary
epithelial cells.
Biological Function of Ribosomal Protein L10 on
Cell Behavior in Human
Epithelial Ovarian
Cancer Jimin Shi, Lingyun Zhang, Daibing Zhou, Jinguo Zhang, Qunbo Lin, Wencai Guan, Jihong Zhang, Weimin Ren, Guoxiong Xu J.
Cancer 2018; 9 (4): 745 - 756.
Differentiation of the mammary
epithelial cell during involution: implications for breast
cancer.
The membrane is coated with proteins that support 3 - D
cell growth and it's seeded with human intestinal
epithelial cells (from a
cell line originally isolated from a colon
cancer).
With the reference
cell census data in hand, the research team is excited to conduct additional studies, including ones involving models or human patients with gastrointestinal conditions — Crohn's disease, ulcerative colitis, gastrointestinal
cancers, forms of food allergy, etc. — aimed at identifying changes in gene expression and
epithelial structure and function that could reveal new insights and opportunities for therapeutic development.
The Intestinal
Epithelial Cell Differentiation Marker ALPi is Selectively Induced by HDAC Inhibitors in Colon
Cancer Cells in a KLF5 - dependent Manner.
Using a qRT - PCR - based method, we found dramatic decreases of
epithelial miRNAs (miR200c and miR205) in the F3 subpopulations compared with the parental breast
cancer cell lines (Figure 6e).
Using cutting - edge techniques enabled by next - generation sequencing, the authors generated complete methylome maps at single nucleotide resolution in a low - passage breast
cancer cell line and normal breast tissue (primary human mammary
epithelial cells).
Loss of PTPN12 Stimulates Progression of ErbB2 - Dependent Breast
Cancer by Enhancing
Cell Survival, Migration, and
Epithelial - to - Mesenchymal Transition.
This unbiased approach revealed global hypomethylation in the
cancer cell line (HCC1954) relative to
epithelial cells (HMEC), and some specific patterns:
Oncologists have suspected for several decades that metastasis usually requires a transition between sticky
epithelial cells, which make up the bulk of solid tumors, and thinner, more mobile mesenchymal
cells that are often found circulating solo in
cancer patients» bloodstreams.
Characterization of a naturally occurring breast
cancer subset enriched in
epithelial - to - mesenchymal transition and stem
cell characteristics.
MUC1 is protein found on
epithelial cells, such as those that line the intestine, that is overexpressed in many
cancers, including colorectal and breast
cancers.
Epithelial - mesenchymal transition (EMT) contributes to the progression of
cancer through enhanced invasion and stem - like properties of
cancer cells.
In human gastric tissue, however, gastric
epithelial cell proliferation is significantly higher but apoptotic indices are lower among persons colonized with cag + compared to cag - strains or uninfected persons leading us to postulate that reduced rates of
cell loss, when accompanied by hyperproliferation, may heighten retention of mutagenized
cells, which could predispose towards
cancer.