Sentences with phrase «epithelial cell culture»

Transient Silencing of 14 -3-3sigma promotes proliferation of p63 - positive progenitor cells isolated from human breastmilk in mammary epithelial cell culture.

Here we show that confluent epithelial cells cultured on finite population - sized domains, exhibit collective low - frequency radial displacement modes as well as stochastic global rotation reversals.

Not exact matches

In cell culture, high expression levels of GPR161 induced proliferation of mammary epithelial cells, disrupted the acinar structures formed by these cells, and enhanced their invasive capacity.

Human nasal epithelial cells, cultured on a microchip, react to air pollutants just like they would in the upper airway.

«We compared the ability of RSV and parainfluenza virus (PIV3)-- another common virus in children that causes much less severe airway disease — to infect and cause inflammatory responses in a cell culture model of human epithelial cells, which compose the lining of the lung airway.

To investigate, a team led by Gerburg Wulf, MD, PhD, a physician scientist in the Hematology / Oncology Division at Beth Israel Deaconess Medical Center (BIDMC) and an Associate Professor of Medicine at Harvard Medical School, cultured breast cells called primary mammary epithelial cells (MECs) in the presence of EBV.

Microscopic images show that RMC - 1 prevented FOXM1 from entering the nucleus in cultured human airway epithelial cells and in mouse allergen - sensitized respiratory airways.

The research team then tested RMC - 1 in cultured human airway epithelial cells and mouse models of asthma (via injection into the animals» peritoneal cavities).

We next attempted to culture TMAdV in an A549 (human lung adenocarcinoma) cell line, a BSC - 1 (African green monkey kidney epithelial) cell line, and PMK (primary rhesus monkey kidney) cells (Fig. 5).

BALB / c corneal epithelial and endothelial cell cultures were established as described previously (23).

This video provides an overview of MimEX ™ Tissue Model Systems, a next - generation 3 - dimensional (3 - D) cell culture platform for the generation of ex vivo epithelial - derived organ tissue.

A549 lung epithelial cells grown as three - dimensional aggregates: alternative tissue culture model for Pseudomonas aeruginosa pathogenesis.

In a first set of experiments, postdoctoral researcher and co-lead author Max - Felix Häring found that insulin increased the proliferation of mouse epithelial tumor cells in culture, as expected.

Syto -59-stained C57BL / 6 CD4 + effector cells were cultured with BALB / c corneal epithelial or endothelial cells overnight.

Thirdly, the current, almost universal dependency of organoid culture on the use of Matrigel as a replacement for the function of the extracellular basement matrix in providing structural support and survival signals to the epithelial cells hampers clinical application, considering its origin from a mouse sarcoma cell line, its poorly defined composition and its mechanical rigidity after plating.

Our Intestine - Chip also allows researchers to culture intestinal epithelial cells alongside other cell types, such as immune cells, and analyze how these different cell types interact.

Laminin - 111 is important during early epithelial development and differentiation of the epiblast, and is commonly used as a general attachment protein in cell culture.

Regulation of inositol 1, 4, 5 - trisphosphate receptor - mediated calcium release by the Na / K - ATPase in cultured renal epithelial cells.

MMTV Env encodes an ITAM responsible for transformation of mammary epithelial cells in three - dimensional culture.

Limbal epithelial stem cells sheets cultured on amniotic membranes for treatment of limbal stem cell deficiency (LSCD).

The primary epithelial cells are expanded as 3D organoids, dissociated, and cultured on a porous membrane within a microfluidic device with human intestinal microvascular endothelium cultured in a parallel microchannel under flow and cyclic deformation.

These epithelial organoid cultures are genetically and phenotypically extremely stable, allowing transplantation of the cultured offspring of a single stem cell, as well as disease modeling by growing organoids directly from diseased patient tissues (45).

Morphogenetic effects of soluble laminin - 5 on cultured epithelial cells and tissue explants.

Mitotic cultured epithelial cell.

Similar to primary keratinocytes, long - term — cultured squamous cell carcinoma (30) and many other epithelial cancer cells (30, 31) can also form different types of clones in culture.

Cellular Component: Limbal epithelial cells Matrix Component: Basement membrane of HAM It is a 4 cm2 denuded human amniotic membrane with cultured autologous limbal biopsy explants with 80 % of the cells positive for p63 marker.

Interestingly, long - term — cultured human epithelial cancer cells in clonal cultures also form holoclones, meroclones, and paraclones, and tumor cell holoclones have been hypothesized to harbor stem - like cells or cancer stem cells.

We also found that several carriage strains had lost their type IV pili and that this loss correlated with reduced tumor necrosis factor alpha (TNF - α) expression when cultured with epithelial cells.

Primary kidney epithelial cells isolated from human proximal tubule are cultured on the upper surface of an extracellular matrix - coated, porous, polyester membrane that splits the main channel of the device into two adjacent channels, thereby creating an apical «luminal» channel and a basal «interstitial» space.

Last year, Mandriota and collaborators demonstrated that in a cancer mouse model, concentrations of aluminum in the amount of those measured in the human breast are able to transform cultured mammary epithelial cells, allowing them to form tumors and to metastasize.

The MCF10A mammary epithelial cells were cultured in DMEM - F12 medium supplemented with 5 % horse serum, 20 ng / ml EGF, 10 μg / ml insulin, 0.5 μg / ml hydrocortisone, 100 ng / ml of cholera toxin, and penicillin / streptomycin.

During the culture of these cells, we noticed a change in cell morphology from the cobble stone - like shape typical of epithelial cells to a more spindle - like and scattered appearance (Figure 2A, lower panel), indicating these cells may be undergoing EMT.

Dienogest, a synthetic progestin, inhibits prostaglandin E (2) production and aromatase expression by human endometrial epithelial cells in a spheroid culture system..