Sentences with phrase «epithelial cell lines»

LARP7 was expressed at relatively high levels in the untransformed mammary epithelial cell lines (MCF10A and EpH4) and noninvasive breast cancer cell lines (MCF7, BT474, T74D, and ZR75B), but was markedly reduced in all four invasive and metastatic cancer lines examined (MDA - MB - 468, BT549, MDA - MB - 231, and MDA - MB - 435; Figure 1D).

We examined the effects of microbe - reactive CD4 + T cells on intestinal stromal and epithelial cell lines.

We are studying these proteins in the intestine and liver in vivo, as well as in epithelial cell lines.

It then travels down the respiratory tract and binds to epithelial cells lining the lung airways via specific molecules on the cell surface.

He determined that epithelial cells lining the brain's blood vessel walls contain a surface protein, or receptor, that can snag insulin, the hormone essential for metabolizing carbohydrates and fats.

The team also analyzed the effects of IL - 10 on epithelial wound closure in vitro using an intestinal epithelial cell line.

Dr. Done explains: «Most breast cancer starts in the epithelial cells lining the mammary ducts.

They found that infection of the narrowest airways of the lung by PIV3 alone caused moderate levels of inflammation, but after infection by PIV3 expressing RSV NS2, the epithelial cells lining the narrow airways were shed rapidly into the airway lumen.

During pregnancy, hormonal signals cause epithelial cells lining the ducts to proliferate and form ball - like structures called alveoli, which is where milk is made when the baby is born.

Studies have found a high - fat diet destroys gut microbiota, reduces the production of epithelial cells lining the intestine and causes gut bacteria to invade intestinal epithelial cells.

Epithelial cells line the cavities and surfaces of structures in the human body.

Differential Response of the Human Renal Proximal Tubular Epithelial Cell Line HK - 2 to Shiga Toxin Types 1 and 2.

Membrane lipidome of an epithelial cell line.

The human mammary epithelial cell line (HuMEC) was maintained in human basal serum - free medium (Life Technologies) with the HuMEC kit (Life Technologies).

Leucine stimulates ASCT2 amino acid transporter expression in porcine jejunal epithelial cell line (IPEC - J2) through PI3K / Akt / mTOR and ERK signaling pathways

The tender epithelial cells lining the chambers of the brain are infected as well.

Not exact matches

Epithelial cells form tissue layers that cover our skin and the inner surfaces of most of our internal organs, while endothelial cells line the adjacent blood - transporting vessels and capillaries that support their functions.

They concluded from that evidence that these specialized RNAs were coming from two types of epithelial cells that line the GI tract.

In a previous study, Professor Schmidt - Ott and his team discovered that it regulates the differentiation of epithelial cells — they line the cavities and surfaces of structures throughout the body — in the mouse embryo.

She and her group had studied the nature and function of epithelial cells, the gut - lining cells that come into contact with breast milk in nursing infants.

So Welham's team set out to engineer vocal fold mucosae in the lab using the two main cell types that make up the tissue — connective fibroblasts, which form the main body, and epithelial cells, which line the surface.

«We compared the ability of RSV and parainfluenza virus (PIV3)-- another common virus in children that causes much less severe airway disease — to infect and cause inflammatory responses in a cell culture model of human epithelial cells, which compose the lining of the lung airway.

«But what we've now shown is that RSV has an increased ability to cause airway obstruction because, during an RSV infection, the virus expresses a specific RSV - encoded non-structural protein, or NS2, in epithelial cells, causing the cells to shed from the airway lining and into the airway lumen.

The research team was also able to shed more light on the role of Paneth cells — a type of cell found in the epithelial lining of the small intestine.

The EMT is a biological process wherein epithelial cells (cells that line the cavities and surfaces of blood vessels and organs) become mesenchymal (skeletal) stem cells that can move throughout the body and differentiate into a variety of cell types.

Digging deeper, the researchers found the inflammatory response through IL - 1β production was initiated in Paneth cells, which are known to protect the intestinal stem cells to replenish the epithelial lining.

«It was known that the epithelial cells which line the airways in the lungs produce a protein called thymic stromal lymphopoietin (TSLP) that causes inflammation.

Epithelial tissue is a sheet of cells that covers a body surface or lines a body cavity.

Different tumor subtypes can include glandular, which include the mammary glands, as well as squamous, which are very rare and involve epithelial cells that line the inside of the breast.

The team's findings may also be relevant to inflammatory disorders that affect other parts of the body, such as the linings of the gut and bowel, which, like the skin, are replenished by epithelial stem cells.

The goal of the first experiment was to see whether lncRNAs are differentially expressed in prostate cancer by measuring total RNA from prostate cancer cell lines and normal epithelial prostatic cells using NCode human ncRNA array and SurePrint G3 human lncRNA microarrays.

The human airway is lined with two kinds of epithelial cells — those that possess hairlike fringes called cilia and those that don't.

The outermost layer of our skin is composed of dead squamous epithelial cells, as are the mucous membranes lining the inside of mouths and body cavities

We next attempted to culture TMAdV in an A549 (human lung adenocarcinoma) cell line, a BSC - 1 (African green monkey kidney epithelial) cell line, and PMK (primary rhesus monkey kidney) cells (Fig. 5).

A549 (human lung adenocarcinoma) and BSC - 1 (African green monkey kidney epithelial) cell lines as well as PMK (primary rhesus monkey kidney) cells are routinely maintained at the Viral and Rickettsial Disease Laboratory (VRDL) branch of the California Department of Public Health.

Previous work by Fredberg and colleagues had documented the importance of collective cellular behavior in asthma, showing that cells comprising epithelial tissues — which line the surfaces of all organs throughout the body — can unjam and flow like a fluid, or jam and freeze like a solid.

First, a hair follicle contains epithelial cells — a cell type that lines the body's vessels and cavities — as well as a specific kind of adult stem cell called dermal papillae.

Every tumor cell line the researchers tested that had the epithelial marker E-cadherin and not the mesenchymal marker Vimentin, had high amounts of miR - 200.

The Joslin team found that intestinal tumors grow just as quickly in a mouse cancer model whose «epithelial» cells lining the inside of the intestine have been genetically modified so that insulin can't activate the cells.

Thirdly, the current, almost universal dependency of organoid culture on the use of Matrigel as a replacement for the function of the extracellular basement matrix in providing structural support and survival signals to the epithelial cells hampers clinical application, considering its origin from a mouse sarcoma cell line, its poorly defined composition and its mechanical rigidity after plating.

It also provides new insights into how the epithelial cells that line the body and its organs stick together.

The membrane is coated with proteins that support 3 - D cell growth and it's seeded with human intestinal epithelial cells (from a cell line originally isolated from a colon cancer).

Scientists taking a close look at how a dangerous food - borne bacterium invades the gut have identified a new culprit, a molecule that normally helps hold the intestinal lining and other sheets of epithelial cells together.

Using a qRT - PCR - based method, we found dramatic decreases of epithelial miRNAs (miR200c and miR205) in the F3 subpopulations compared with the parental breast cancer cell lines (Figure 6e).

Using cutting - edge techniques enabled by next - generation sequencing, the authors generated complete methylome maps at single nucleotide resolution in a low - passage breast cancer cell line and normal breast tissue (primary human mammary epithelial cells).

In the Intestine Chip, the epithelium forms villi - like projections lined by polarized epithelial cells that undergo multi-lineage differentiation similar to that of intestinal organoids, however, these cells expose their apical surfaces to an open lumen and interface with endothelium.

Normal breast tissue showed acini lined by epithelial cells resting on an intact myoepithelial cell layer (Figure 1A).

This unbiased approach revealed global hypomethylation in the cancer cell line (HCC1954) relative to epithelial cells (HMEC), and some specific patterns:

Condensin II and GAIT Complexes Cooperate to Restrict LINE - 1 Retrotransposition in Epithelial Cells.

«Also in real life, organs have an epithelial lining, a vascular component, connective tissues and immune cells, none of which can yet be replicated in their normal physical arrangement in organoids,» says Donald Ingber, founding director of the Wyss Institute for Biologically Inspired Engineering at Harvard University.