Sentences with phrase «epithelial stem cells»

Using Cultrex ® BME, MimEX ™ Tissue Models System provides a platform for the isolation, expansion, and differentiation of adult epithelial stem cells into 3 - D ex vivo organ tissue.

In the Xu study, the team's protocol succeeded in turning over 25 % of the iPSCs into epithelial stem cells in 18 days.

This protocol video describes how to thaw and expand adult epithelial stem cells in the MimEX ™ System.

That said, iPSC - derived epithelial stem cells are not yet ready for use in human subjects, Xu adds.

A membrane — designed to support the cultivation and differentiation of human nasal epithelial stem cells — was inserted into a small chamber on the device and fresh or contaminated air was fed through a tiny channel.

Her group is particularly interested in the cellular and molecular mechanisms which control lung epithelial stem cell fate decisions.

Limbal epithelial stem cells sheets cultured on amniotic membranes for treatment of limbal stem cell deficiency (LSCD).

Three years after publishing a groundbreaking paper in which she isolated adult retinal pigment epithelial stem cells, Temple is in preclinical trials with rats.

Their work demonstrate the close interaction between epithelial stem cells and the niche that regulates them.

The team's findings may also be relevant to inflammatory disorders that affect other parts of the body, such as the linings of the gut and bowel, which, like the skin, are replenished by epithelial stem cells.

This distribution helps to maintain the health and rapid proliferation rate of adult epithelial stem cells using MimEX ™ Tissue Model Systems.

A new study by Reichenbach et al (2018) reports coordinated proliferation of three distinct epithelial stem cell niches and a role for glutamate transporter Slc1a3 in synchronisation of activated stem and progenitor cells.

MimEX ™ Tissue Model Systems harnesses the unique characteristics of adult ground - state epithelial stem cells to generate ex vivo organ tissue that recapitulates native cytoarchitecture and mimics physiological attributes of the desired organ.

The stem cells in the bulge are multipotent epithelial stem cells, and can become, or differentiate into, all the epithelial cell types in the follicle (including hair follicles, epidermis, and sebaceous glands)(Oshima et al., 2001).

However, our recent achievements in human gastric epithelial stem cell cultivation now enables a more detailed characterization of the progenitors of the well - described ascending tissue pathologies culminating in cancer.

Interestingly, we detected upregulation of p16 and p15 in Polycomb - null skin epithelial stem cells in young mice where it is normally detected only in aged animals.

Differentiation - related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells.

Existence of slow - cycling limbal epithelial basal cells that can be preferentially stimulated to proliferate: implications on epithelial stem cells.

Creating human lung epithelial cells in the lab has been a challenge, and lineage - specific reporters, which indicate each cell's specific type, are key to understanding lung epithelial stem cell development.

This shows hair shafts (arrows) formed by induced pluripotent stem cell - derived epithelial stem cells.

They then converted the iPS cells into epithelial stem cells, normally found at the bulge of hair follicles.

Five weeks after seeding the membrane with human nasal epithelial stem cells the researchers could observe the formation of beating cilia.

Limbal epithelial stem cells (LESCs) reside in the basal layer of the limbal area, at the vascularised junction between the corneal and conjunctival epithelium.

MimEX ™ Tissue Models System provides a platform for the isolation, expansion, and differentiation of adult epithelial stem cells into 3 - D ex vivo organ tissue.

«This is the first time anyone has made scalable amounts of epithelial stem cells that are capable of generating the epithelial component of hair follicles,» Xu says.

Starting with procedures other research teams had previously worked out to convert iPSCs into keratinocytes, Xu's team demonstrated that by carefully controlling the timing of the growth factors the cells received, they could force the iPSCs to generate large numbers of epithelial stem cells.

The epithelial stem cells, when implanted into immunocompromised mice, regenerated the different cell types of human skin and hair follicles, and even produced structurally recognizable hair shaft, raising the possibility that they may eventually enable hair regeneration in people.

Comparison of the gene expression patterns of the human iPSC - derived epithelial stem cells with epithelial stem cells obtained from human hair follicles showed that the team had succeeded in producing the cells they set out to make in the first place.

Xiaowei «George» Xu, MD, PhD, associate professor of Pathology and Laboratory Medicine and Dermatology at the Perelman School of Medicine, University of Pennsylvania, and colleagues published in Nature Communications a method for converting adult cells into epithelial stem cells (EpSCs), the first time anyone has achieved this in either humans or mice.

First, the MimEX ™ Cell Plating Procedure is used to maintain a uniform irradiated fibroblast monolayer for the rapid clonal expansion and efficient differentiation of tissue - specific adult epithelial stem cells.

It involves isolation and propagation of adult epithelial stem cells, prepared from tissue specimens at different early stages of chronic and atrophic gastritis, as well as from the late, premalignant (irreversible) stage of metaplasia.

For example, ΔNp63α is expressed in epithelial stem cells, while TAp63α is expressed in fully differentiated cell types.