Sentences with phrase «epitope tags»
Epitope tags refer to small, specific protein tags that are attached to another protein of interest. These tags help scientists identify, purify, or visualize the protein they are interested in studying. They act like a label or a beacon, enabling researchers to easily detect and manipulate the tagged protein. Full definition
Because of these limitations, the AAV approach is best suited for tissue culture based approaches that require modification of only a single allele of a gene, such as the introduction of heterozygous endogenous epitope tags, or the introduction of dominant mutations of a gene into the endogenous allele in cultured cells.
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Kim, Jung - Sik, et al. «Epitope tagging of endogenous genes in diverse human cell lines.»
Some examples from their lab include using AAV to introduce epitope tags into the endogenous alleles of the p53 and PTEN tumor suppressor genes in human cells (Kim et al 2008).
Modern Cytological Approaches Including Epitope Tagging and Imaging Yeast Cells Using Fluorescence Microscopy
The CRISPR / Cas9 system is a tremendously versatile tool for engineering a wide variety of genetic alterations including knockouts (KO), deletions, point mutations, and short insertions (e.g. loxP sites, epitope tags, etc.).
The expression vector contained the cDNAs coding for a potential fusion protein containing the GFP (EGFP), the 2A esterase of foot - and - mouth disease virus (FMDV), and HOXB4 with an additional N - terminal hemagglutinin (HA) epitope tag (eGFP2AHOXB4).