Differentiation of this heterozygous iPSC line to
erythrocytes used an improved feeder - free and xeno - free three - step culture strategy, generating enucleated hemoglobin expressing cells to a similar level to that observed for the parental TNC1 iPSC line (see figure).
My particular field of expertise (or more correctly, least incompetence) was investigating interactions of the lithium ion with
erythrocytes using nuclear magnetic resonance (NMR) spectroscopy.
Not exact matches
AppleActiv has been tested for its antioxidant content
using industry standard antioxidant tests such as the CAP - e (Cellular Antioxidant Protection in
erythrocytes).
The effects of the dietary intervention, mode of feeding (breast - fed or formula - fed), and time were evaluated by
using three - factor repeated - measures ANOVA for the outcomes of reported number of egg yolks consumed; reported consumption of meat, chicken, and fish; reported consumption of baby cereal; reported consumption of adult cereal;
erythrocyte DHA and AA; plasma cholesterol; and indexes of iron status.
The turkey
erythrocyte model system was
used earlier to show that a covalent modification of the receptor (phosphorylation) is associated with this process.
The work highlights the importance of fibrinogen -
erythrocyte interactions in patients with chronic heart disease, and our technique can be
used to identify patients at higher risk for this disease,» he tells nanotechweb.org.
So say researchers in Lisbon, Portugal, who have
used the technique to show that the interactions between a protein known as fibrinogen and
erythrocytes (red blood cells) are modified in people with chronic heart failure.
Now, Santos and colleagues have
used atomic force microscopy to measure the binding forces between fibrinogen and
erythrocytes.
«
Using the tip of an atomic force microscope (AFM), we have found that the mechanical force required to break the bond between fibrinogen and
erythrocytes is higher in patients with chronic heart failure than in healthy subjects,» explains team leader Nuno C Santos from iMM and the Faculty of Medicine at the University of Lisbon.
«
Using an AFM in «force spectroscopy» mode, we in fact
used a single fibrinogen molecule as a «bait» to «fish» its receptor on the
erythrocytes» surface,» says Santos.
Erythrocyte, white blood cell, and platelet counts; hemoglobin concentrations; mean cell volumes; and mean cell hemoglobin concentrations were measured with the
use of a Coulter automated cell counter (MAX - M; Beckman Coulter).
CRP values can be
used much like an older test, the
erythrocyte sedimentation rate (ESR).