Sentences with phrase «established cell line»

It uses an established cell line that yields a vaccine with consistent potency.

As well as allowing the use of stem cells grown from established cell lines, the technology could enable the creation of improved human tissue models for drug testing and potentially even purpose - built replacement organs.

«We learned this in the 70s when investigators found that HeLa cells had contaminated many established cell lines and we have to relearn it every so often: you have to authenticate your cells.

Established cell lines exhibited several inherent endothelial properties, including the expression of constitutive and inducible levels of endothelial cell adhesion molecules E-selectin, intercellular adhesion molecule - 1, and vascular cell adhesion molecule - 1, internalization of acetylated low - density lipoprotein, and formation of loop structures on Matrigel surfaces.

The researchers established cell lines from the original egg and in seven out of eight cases the amount of mutated mitochondrial DNA remained low when the cells were grown in the laboratory.

Lentiviruses are a powerful laboratory tool often employed to establish cell lines that stably express a gene of interest.

Although the differing effects of hypoxia reported here are in conflict with previously published data, where no contrasting effect was seen (18 — 20), our data was produced using 13 primary breast cancer samples, as well as established cell lines, and the opposing effects in hormone receptor — positive and negative tumors, measured using multiple in vitro and in vivo CSC assays, were consistent in all cases.

Not exact matches

Honda established a division late last year to develop electric vehicles (EVs) as part of its long - held goal for lower - emission gasoline hybrids, plug - in hybrids, EVs and hydrogen fuel cell vehicles (FCVs) to account for two - thirds of its line - up by 2030, from about 5 percent now.

«Brain scientists have invented, established and patented a method to obtain long - living cell lines derived from fungiform taste papillae of the human tongue.

Before we established in - house human taste cell (HTC) technologies, it was not possible to use human tongue - derived cells as a model, due to the lack of homogenous, proliferating cell lines with defined properties, which is a prerequisite to establish comprehensive research and screening programs.

This is a breast cancer stem cell line 1 (BCSC1) from the newly established cell model.

Henrietta Lacks famously (and unwittingly) established the HeLa cell line, one of the most widely used lines in cancer research.

Using genetic analyses, the researchers showed that a commonly used cell line which was established in Uppsala, Sweden, almost fifty years ago does not originate from the patient it is claimed to stem from.

Researchers studying the brain tumor type glioma often use a cell line called U87MG that was established at Uppsala University almost fifty years ago.

Many scientific journals require researchers who report results based on cell line experiments to use DNA profiling to establish the identity of the used cells.

When the cell line was established in the 1960s, material from the original tumor was saved as thin sections on microscope slides.

Using a very sensitive DNA analysis technique that can also be employed when only very small amounts of DNA from old tissue are available, the researchers could compare the two current cell lines with the tumor from which the cell line was established.

The arrangement establishes a precedent in cell line research ethics, granting Lacks a new legacy — itself a kind of immortality.

Before this work, no one knew whether cell lines could be established using nuclei donated from men or young girls.

Moreover, Albertini's group is exploring whether these plumbing lines, which corkscrew into the outer rind of the egg, play a role in establishing one of the most important geographic landmarks in the life of an egg cell — an event, Albertini likes to say when lecturing medical students, that marks «one of the most important days in your life.»

Elsewhere in the United Kingdom, researchers at the University of Sheffield and Roslin Cells, a company based in Edinburgh, are also establishing clinical - grade lines.

The university recruited him with one of the first awards for established investigators from the Cancer Prevention and Research Institute of Texas (CPRIT), a $ 3 billion, 10 - year cancer research fund along the lines of California's stem cell initiative.

In other cases, the goal was to establish a line of embryonic stem cells.

On 9 August, President George W. Bush announced that federal funding of stem cell research is permitted, but only for that on cell lines already established from embryos discarded by fertility clinics by the time of his statement.

As famously chronicled in science writer Rebecca Skloot's best - selling book, the cell line was established, without Lacks's consent, from a cervical tumor that she was diagnosed with in 1951.

Each of the endothelial cell lines established from the H - 2Kb - tsA58 transgenic mice was capable of generating vascular - like channels on Matrigel within 12 h.

We used this paradigm following the field of neurodegenerative disorders associated to protein misfolding, in which a widely established method for studying propagation of protein aggregates in vitro consists of exposing cell lines to specific protein aggregates.

The immortalized endothelial cell lines established from H - 2Kb - tsA58 mice provide, for the first time, a cell culture system to examine factors regulating angiogenesis and tumor cell arrest in different organ systems.

He is part of the pioneering groups which established human ES cell lines and distributed them to other scientists free of charge.

The authors studied a standard panel of 60 established human tumor cell lines representing nine different human cancers, as well as several specimens of human primary ovarian cancer.

The original cell line was established in Uppsala almost fifty years ago.

Due to the high efficiency of establishing organoid models from different tissues and diseases, such as cancer, organoid technology allows the generation of large living biobanks of tumor organoids that are amenable for middle - throughput drug screens and may allow personalized therapy design, as a complement to cell line and xenograft - based drug studies (7,19).

Establishing standards for ESC media and cell lines «lays the foundation for moving toward [good manufacturing practice],» adds Derek Hei, director of the US National Stem Cell Bank in Madison, Wicell lines «lays the foundation for moving toward [good manufacturing practice],» adds Derek Hei, director of the US National Stem Cell Bank in Madison, WiCell Bank in Madison, Wisc..

One caveat is that the authors also observed that one of the eight human embryonic stem cell lines established following spindle transfer showed a drift in mitochondrial heteroplasmy in a subset of stem cells, despite low levels of carryover detected in the embryo.

Flpe - and Cre - mediated recombination in vitro generated ES cell lines that contained a conditional or null Sema3B allele, respectively, which were established as homozygous alleles in mice.

We then isolated and exposed this novel subpopulation to additional cycles of hypoxia / reoxygenation and established a distinct subpopulation of cells from these two breast cancer cell lines.

We have established stably transfected HEK 293 cell lines expressing high levels of functional human ether - a go - go - related gene (HERG) channels.

This is the route our project is pursuing, but it may take several years to establish a cultured cell line; bird primordial germ cells are quite finicky about living outside of a bird's body.

Transgenes are inactivated and the endogenous Oct4 promoter is completely demethylated in the established iPS cell lines, indicating a fully reprogrammed state.

The cell lines established in this study are freely available for the scientific community.

In this study, we established mouse iPS cell lines from NPCs of neonatal EGFP - transgenic mice on the HFF feeder cells via viral transduction of 4 factors, combined with application of 2i.

The first step in using stem cells for disease treatment is to establish stem cell lines, which researchers have accomplished.

The third interesting advance made in this study is the discovery that mouse iPS cell lines could be established and maintained for 30 passages on HFF cells without exogenous LIF.

In an executive order, Bush stated that federal funds could only be used for research on human embryonic stem cell lines that had already been established (only 22 cell lines).

We established several cell lineages from three independent transfection experiments and chose three lines (iPSC1, iPSC2, iPSC3) for further characterization.

We have established HFF cell lines which stably express resistant genes against neomycin, puromycin and hygromycin (data not shown).

Most prior established iPS cell lines were derived and maintained on mouse embryonic fibroblast (MEF) cells supplemented with exogenous leukemia inhibitory factor (LIF).

Tang wondered if he could use previously established mouse lines expressing GFP in specific cell types to do more than just label cells.

To further investigate the role of STAT6 in regulating constitutive COX - 2 expression, a deleted form of STAT6 lacking the C - terminal 186 amino acids (aa)(from 662 - 847, MW 75 kDa) that has been shown to act dominant - negatively (STAT6 DN) was constructed and stable cell lines were established (Mikita et al., 1996).

Therefore, it is necessary to establish multiple independent iPS cell lines from individual donors to ensure efficient generation of various types of functional cells for future application in the treatment of diseases.