Sentences with phrase «established cell lines»

As well as allowing the use of stem cells grown from established cell lines, the technology could enable the creation of improved human tissue models for drug testing and potentially even purpose - built replacement organs.

Gb3 expression in established cell lines of gastric carcinoma was heterogeneous, with 6 of 10 lines being positive, evidenced by flow cytometry.

Fetal tissue, from spontaneous or induced abortions, is particularly useful for establishing cell lines and cultures.

«We learned this in the 70s when investigators found that HeLa cells had contaminated many established cell lines and we have to relearn it every so often: you have to authenticate your cells.

Established cell lines exhibited several inherent endothelial properties, including the expression of constitutive and inducible levels of endothelial cell adhesion molecules E-selectin, intercellular adhesion molecule - 1, and vascular cell adhesion molecule - 1, internalization of acetylated low - density lipoprotein, and formation of loop structures on Matrigel surfaces.

Potentially Hazardous Biological Agents (6A) This form is filled out by the student researcher and is required for all research involving microorganisms, rDNA and fresh / frozen tissue (including primary cell lines, human and other primate established cell lines and tissue cultures), blood, blood products, and body fluids.

The researchers established cell lines from the original egg and in seven out of eight cases the amount of mutated mitochondrial DNA remained low when the cells were grown in the laboratory.

Although the differing effects of hypoxia reported here are in conflict with previously published data, where no contrasting effect was seen (18 — 20), our data was produced using 13 primary breast cancer samples, as well as established cell lines, and the opposing effects in hormone receptor — positive and negative tumors, measured using multiple in vitro and in vivo CSC assays, were consistent in all cases.

As patient advocate at the University of Colorado Cancer Center Lung Cancer Specialized Program of Research Excellence (SPORE), Freeman - Daily spoke with Robert C. Doebele, MD, PhD, CU Lung SPORE PI and Director of CU Cancer Center Thoracic Oncology Research Initiative, and in partnership, CU, the Global ROS1 Initiative and the Bonnie J. Addario Lung Cancer Foundation created a program in which patients could donate cancer tissue for use in establishing cell lines.

Lentiviruses are a powerful laboratory tool often employed to establish cell lines that stably express a gene of interest.

It uses an established cell line that yields a vaccine with consistent potency.