Sentences with phrase «established mouse lines»
Tang wondered if he could use previously established mouse lines expressing GFP in specific cell types to do more than just label cells.
http://blog.addgene.org/ Not exact matches
The consortium had to establish new standardized procedures to generate and assess the mouse lines and a central European database to store all the data.
These mouse lines were then analyzed in mouse clinics, where each line was assessed by a series of tests and investigations, allowing to establish the role of the missing genes.
Each of the endothelial cell lines established from the H - 2Kb - tsA58 transgenic mice was capable of generating vascular - like channels on Matrigel within 12 h.
I was able to establish a transgenic line from one of these founders (F66), and I backcrossed this line extensively to C57 BL / 6 mice for subsequent analysis.
The immortalized endothelial cell lines established from H - 2Kb - tsA58 mice provide, for the first time, a cell culture system to examine factors regulating angiogenesis and tumor cell arrest in different organ systems.
Flpe - and Cre - mediated recombination in vitro generated ES cell lines that contained a conditional or null Sema3B allele, respectively, which were established as homozygous alleles in mice.
In this study, we established mouse iPS cell lines from NPCs of neonatal EGFP - transgenic mice on the HFF feeder cells via viral transduction of 4 factors, combined with application of 2i.
The third interesting advance made in this study is the discovery that mouse iPS cell lines could be established and maintained for 30 passages on HFF cells without exogenous LIF.
Most prior established iPS cell lines were derived and maintained on mouse embryonic fibroblast (MEF) cells supplemented with exogenous leukemia inhibitory factor (LIF).
His pioneering mouse embryonic stem (ES) cell work in the 1980s, demonstrating germ - line transmission and the great potential of ES cells to generate mice carrying mutations in endogenous genes, established milestones in a field that saw the award of the 2007 Nobel Prize in Physiology or Medicine to Mario Capecchi, Martin Evans, and Oliver Smithies.
Genomic (in) stability: We have established several cell lines and mice carrying null mutations for specific DNA repair functions.
The CD8 + or CD4 + T cell lines were established in vitro by stimulating splenocytes from Gasp + / + and Gasp − / − mice after depleting CD4 + or CD8 + cells, with BALB / c (allogeneic) splenocytes or syngeneic splenocytes in the presence of 2C11 (1 μg / mL).
Appropriate requirements for REALTORS ® establishing contractual relationships with consumers on - line (e.g. should more than a «mouse click» be required?)