Light microscopic evaluation of histological and histochemical stains and
reactions was performed according to standard protocols [20] and included hematoxylin and eosin, modified Gomori trichrome, periodic acid Schiff, phosphorylase,
esterase, myofibrillar ATPase
reaction at preincubation pH of 9.8, 4.5, and 4.3, reduced nicotinamide adenine dinucleotide - tetrazolium reductase, succinic dehydrogenase, acid phosphatase, and oil red O.