There was a good correlation with
esterase staining and AChR labeling in the normal dog.
In the Labrador Retriever with CMS,
esterase staining was evident in multiple locations but did not fully correlate with AChR labeling (Figure 4).
In the CMS Jack Russell Terrier
esterase staining was present; however, staining for AChR was markedly decreased or absent, consistent with a markedly decreased AChR content.
Not exact matches
Serial sections were evaluated with light microscopy (
esterase reaction) and fluorescent microscopy (red fluorescence) and localization of
stainings compared.
In the Jack Russell Terrier with CMS (neuromuscular disease control), several end - plates were
stained with
esterase; however, no AChRs were labeled in the serial muscle section, consistent with the marked decrease in muscle AChRs described in this breed.
Multifocal areas of
esterase reactivity were identified, but it could not be determined from this reaction if
staining correlated with localization of motor end - plates.
Light microscopic evaluation of histological and histochemical
stains and reactions was performed according to standard protocols [20] and included hematoxylin and eosin, modified Gomori trichrome, periodic acid Schiff, phosphorylase,
esterase, myofibrillar ATPase reaction at preincubation pH of 9.8, 4.5, and 4.3, reduced nicotinamide adenine dinucleotide - tetrazolium reductase, succinic dehydrogenase, acid phosphatase, and oil red O.
For each dog, histochemical
staining for
esterase activity (brown
stain) is shown along with a serial section demonstrating immunofluorescent localization of α - bungarotoxin for AChR and end - plate localization (red color).