Sentences with phrase «ethanol precipitation»
"Ethanol precipitation" refers to a technique used to separate and extract specific substances from a mixture by adding ethanol to the mixture. The ethanol causes the desired substance to separate and form visible clumps or solids, which can then be collected and further processed. Full definition
This is the case for DNA concentration, which is generally achieved via ethanol precipitation followed by analysis on electrophoresis gel.
sciencedaily.com
Standard laboratory DNA isolation methods, such as sonication, ribonuclease (RNase) treatment, and ethanol precipitation may reduce the available pool of DNA enough to prevent recovering usable sequences from the oldest samples.
Following phenol / chloroform extraction and ethanol precipitation, the cDNA was transcribed into biotin - labeled cRNA using T7 polymerase (Ambion MEGAScript T7).
To generate a full - length unc - 9:: gfp construct, the 14.7 - kb UNC - 9A + B PCR product (representing the 5» end of unc - 9) and the unc - 9:: gfp plasmid were digested separately with Kpn I, digests electrophoresed through low - melt agarose (SeaPlaque GTG agarose, FMC, Rockland, ME, USA), and appropriate fragments purified by phenol extraction and ethanol precipitation.