To determine how tightly the probe binds, researchers heat it up to shake the probe loose; however, often there's only a few degrees difference between the «melting» temperature of a probe bound to
the exact target sequence and one where there is a minor discrepancy.
Not exact matches
They identified ALK - positive mesotheliomas by immunohistochemistry; confirmed with fluorescence in situ hybridization; and performed
targeted next - generation
sequencing of tumor DNA and RNA to get a full picture of the
exact genetic rearrangement underpinning the disease.
It became possible to design FLEx vectors (Flip - excision vectors) that only allow one inversion event when it was discovered that it is not the
exact sequence of the asymmetric
target site core but its 8 bp length that is critical for Cre and FLP function.