Sentences with phrase «excitation wavelength»

Amrose (a portmanteau of American rose) remains with ∼ 30 % of the peak maximum highly excitable at 633 nm, an often used excitation wavelength of optical - imaging instruments, and when expressed in DT40 demonstrates functionality in the whole - mouse imaging model at 670 nm excitation.

The fluorescence lifetimes and the quantities of the components with different lifetimes of a defined protein are independent from the used excitation wavelength.

High - power LEDs are now available in a wide range of emission bands, allowing for the matching of excitation wavelength with a variety of potential fluorophores.

Time resolved fluorescence measurements were performed at three different excitation wavelengths, i.e., 525, 565, and 595 nm, respectively and read out at 645 nm for the Amrose variants and eqFP650 with the time - resolved fluorometer FLS 920 from Edinburgh Instruments.

Amrose v3 visualized in zebrafish 24 hours after 250 ng / µl mRNA injection at 561 nm, 594 nm and 633 nm excitation wavelengths.

The shifts in emission peaks are significant; however, absorption shows little to no spectral shift, presumably due to the ratio sorting protocol used at a constant excitation wavelength of 568 nm.

When FRET donor excitation wavelengths overlap with optogenetic initiation wavelengths, excitation of the optogenetic regulator leads to spurious activation of the FRET sensor.

The instrument response function (IRF) was measured and monitored at the respective excitation wavelengths using a scattering solution.

The practical brightness takes all application specific parameters into account, including the specs of your microscope (excitation wavelength, available emission filters, and detector sensitivity) and the biological system (temperature, prokaryote versus eukaryote, background fluorescence).

Nuclei were stained with Hoechst 33342 (5 μg / ml) and observed by a fluorescence microscope equipped with U-MWU optical filters with a U-MWU optical filter at an excitation wavelength of 355 nm and an emission wavelength of 420 nm.

λex - excitation wavelength, λem - emmission wavelength, τ1 - fluorescence lifetime 1, B1 - pre-exponential factor 1, χ2 - fit quality parameter.

Here, f (λex) is the absorption factor at the excitation wavelength λex, F the integral emission intensity, i.e., the area under the blank and spectrally corrected emission spectrum on a wavelength scale, and n the refractive index of the solvent (s) used.

Image recording was performed with an excitation wavelength of 633 nm to scan the respective emission profiles and recording the emission in 10 nm windows from 660 to 780 nm.

Intensity as recorded in zebrafish (crosshairs in Fig. 4a show where measurement took place) at an excitation wavelength of 633 nm and scanned for emission at 11 nm intervals from 654 nm to 794 nm.

First, the tubes were imaged in reflectance mode against a dark background for a series of excitation wavelengths and emission filters to establish a baseline of fluorescent protein expression.

Traditional FRET reporters could not be used as the excitation wavelengths needed would also activate the optogenetic accuators.

Consistent with spectroscopy measurements, Amrose v3 can be efficiently excited with the commonly used yellow (561, 594 nm) or red laser (633 nm) excitation wavelengths (Figure 5) as demonstrated here by image recording of the embryonic retina or skeletal muscle in the trunk.

Fluorescence intensities were measured at an excitation wavelength of 340 or 380 nm and an emission wavelength of 510 nm, with a fluorescence spectrometer (Hitachi F - 2500) during stimulation as indicated in Fig. 5.