Sentences with word «exonuclease»

DNA polymerase 1 and d exonuclease domain mutations in endometrial cancer.

Enzymes called exonucleases may contribute to aging by chewing up the ends of chromosomes.

DNA had unraveled disproportionately at the exposed ends of the chromosomes, which is what they'd expect to see if exonuclease damage was the first step in genetic instability.

The researchers suspected that early damage from exonucleases might be going unnoticed because the uncapped ends of chromosomes fuse soon afterward.

A single - stranded, 3» extension of three nucleotides bound to the 3» to 5» exonuclease active site.

Cells lacking a particular exonuclease, called Exo1p, didn't display unraveled DNA at the chromosome tips, the researchers report in the December issue of Molecular and Cellular Biology.

The TREX2 gene codifies exonuclease that was related to the maintenance of genomic stability, and certain repairmen mechanisms such as DNA degradation.

Biallelic mutations in the 3» exonuclease TOE1 cause pontocerebellar hypoplasia and uncover a role in snRNA processing.

We have four major research programs on: (a) understanding the polarization machinery of mammalian epithelial cells (b) control of epithelial homeostasis in mammary gland morphogenesis, breast cancer initiation and metastasis (c) the role of stem cells and mechanisms controling stemness in the mammary gland and in breast cancer (d) the role of a novel exonuclease in DNA repair of inter-strand crosslinks.

Structural Basis for the dsRNA Specificity of the Lassa Virus NP Exonuclease.

Products were purified using the Qiax II Gel Extraction Kit (Qiagen) or an ExoSAP master mix consisting of 0.5 units of Exonuclease I (New England BioLabs) and 0.25 units of SAP (Promega).

When the fragment bound DNA, a region previously referred to as the «disordered domain» became more ordered and moved along with two helices toward the 3» to 5» exonuclease domain to form the binding groove.

(Earlier work by David Lydell and Laura Maringele at the University of Manchester, U.K., hinted that an exonuclease could chew up telomeres.)

Although this cocrystal structure appears to be an editing complex, it suggests that the primer strand approaches the catalytic site of the polymerase from the direction of the 3» to 5» exonuclease domain and that the duplex DNA product may bend to enter the cleft that contains the polymerase catalytic site.

Greider and Hackett wondered whether short telomeres might render chromosomes vulnerable to a type of enzyme called an exonuclease, which degrades DNA.

Klenow fragment of Escherichia coli DNA polymerase I, which was cocrystallized with duplex DNA, positioned 11 base pairs of DNA in a groove that lies at right angles to the cleft that contains the polymerase active site and is adjacent to the 3» to 5» exonuclease domain.

The Escherichia coli methyl - directed reaction has been reconstituted in a purified system consisting of MutH, MutL, and MutS proteins, DNA helicase II, single - strand DNA binding protein, DNA polymerase III holoenzyme, exonuclease I, DNA ligase, along with ATP (adenosine triphosphate), and the four deoxynucleoside triphosphates.

Exonuclease 1 is essential for maintaining genomic stability and the proliferative capacity of neural but not hematopoietic stem cells.